Mechanosensing machinery for cells under low substratum rigidity

Wei Chun Wei, Hsi Hui Lin, Meng Ru Shen, Ming Jer Tang

研究成果: Article同行評審

63 引文 斯高帕斯(Scopus)

摘要

Mechanical stimuli are essential during development and tumorigenesis. However, how cells sense their physical environment under low rigidity is still unknown. Here we show that low rigidity of collagen gel downregulates β1-integrin activation, clustering, and focal adhesion kinase (FAK) Y397 phosphorylation, which is mediated by delayed raft formation. Moreover, overexpression of autoclustered β1-integrin (V737N), but not constitutively active β1-integrin (G429N), rescues FAKY397 phosphorylation level suppressed by low substratum rigidity. Using fluorescence resonance energy transfer to assess β1-integrin clustering, we have found that substratum rigidity between 58 and 386 Pa triggers β1-integrin clustering in a dose-dependent manner, which is highly dependent on actin filaments but not microtubules. Furthermore, augmentation of β1-integrin clustering enhances the interaction between β1-integrin, FAK, and talin. Our results indicate that contact with collagen fibrils is not sufficient for integrin activation. However, substratum rigidity is required for integrin clustering and activation. Together, our findings provide new insight into the mechanosensing machinery and the mode of action for epithelial cells in response to their physical environment under low rigidity.

原文English
頁(從 - 到)C1579-C1589
期刊American Journal of Physiology - Cell Physiology
295
發行號6
DOIs
出版狀態Published - 2008 12月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 細胞生物學

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