Mercury-induced biochemical and proteomic changes in rice roots

Yun An Chen, Wen Chang Chi, Tsai Lien Huang, Chung Yi Lin, Thi Thuy Quynh Nguyeh, Yu Chywan Hsiung, Li Chiao Chia, Hao Jen Huang

研究成果: Article同行評審

57 引文 斯高帕斯(Scopus)

摘要

Mercury (Hg) is a serious environmental pollution threats to the planet. Accumulation of Hg in plants disrupts many cellular-level functions and inhibits growth and development, but the mechanism is not fully understood. We investigated cellular, biochemical and proteomic changes in rice roots under Hg stress. Root growth rate was decreased and Hg, reactive oxygen species (ROS), and malondialdehyde (MDA) content and lipoxygenase activity were increased significantly with increasing Hg concentration in roots. We revealed a time-dependent alteration in total glutathione content and enzymatic activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) during Hg stress. 2-D electrophoresis revealed differential expression of 25 spots with Hg treatment of roots: 14 spots were upregulated and 11 spots downregulated. These differentially expressed proteins were identified by ESI-MS/MS to be involved in cellular functions including redox and hormone homeostasis, chaperone activity, metabolism, and transcription regulation. These results may provide new insights into the molecular basis of the Hg stress response in plants.

原文English
頁(從 - 到)23-32
頁數10
期刊Plant Physiology and Biochemistry
55
DOIs
出版狀態Published - 2012 6月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 遺傳學
  • 植物科學

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