Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis

Chung Chih Tseng, Yu Ju Lin, Wangta Liu, Hao Yeh Lin, Hsin Yu Chou, Caroline Thia, Jou Hsien Wu, Jo Shu Chang, Zhi Hong Wen, Jui Jen Chang, Hui Min David Wang

研究成果: Article

摘要

3S, 3′S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3′S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3′S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.

原文English
文章編號110993
期刊Food and Chemical Toxicology
135
DOIs
出版狀態Published - 2020 一月

指紋

Metabolic engineering
Metabolic Engineering
metabolic engineering
astaxanthin
Probiotics
metastasis
Yeast
probiotics
Yeasts
Neoplasm Metastasis
yeasts
Monophenol Monooxygenase
Biosynthesis
biosynthesis
Food Additives
biosafety
Biochemistry
Body Weight Changes
blood chemistry
Cosmetics

All Science Journal Classification (ASJC) codes

  • Food Science
  • Toxicology

引用此文

Tseng, C. C., Lin, Y. J., Liu, W., Lin, H. Y., Chou, H. Y., Thia, C., ... David Wang, H. M. (2020). Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis. Food and Chemical Toxicology, 135, [110993]. https://doi.org/10.1016/j.fct.2019.110993
Tseng, Chung Chih ; Lin, Yu Ju ; Liu, Wangta ; Lin, Hao Yeh ; Chou, Hsin Yu ; Thia, Caroline ; Wu, Jou Hsien ; Chang, Jo Shu ; Wen, Zhi Hong ; Chang, Jui Jen ; David Wang, Hui Min. / Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis. 於: Food and Chemical Toxicology. 2020 ; 卷 135.
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abstract = "3S, 3′S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3′S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3′S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.",
author = "Tseng, {Chung Chih} and Lin, {Yu Ju} and Wangta Liu and Lin, {Hao Yeh} and Chou, {Hsin Yu} and Caroline Thia and Wu, {Jou Hsien} and Chang, {Jo Shu} and Wen, {Zhi Hong} and Chang, {Jui Jen} and {David Wang}, {Hui Min}",
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Tseng, CC, Lin, YJ, Liu, W, Lin, HY, Chou, HY, Thia, C, Wu, JH, Chang, JS, Wen, ZH, Chang, JJ & David Wang, HM 2020, 'Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis', Food and Chemical Toxicology, 卷 135, 110993. https://doi.org/10.1016/j.fct.2019.110993

Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis. / Tseng, Chung Chih; Lin, Yu Ju; Liu, Wangta; Lin, Hao Yeh; Chou, Hsin Yu; Thia, Caroline; Wu, Jou Hsien; Chang, Jo Shu; Wen, Zhi Hong; Chang, Jui Jen; David Wang, Hui Min.

於: Food and Chemical Toxicology, 卷 135, 110993, 01.2020.

研究成果: Article

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AU - Tseng, Chung Chih

AU - Lin, Yu Ju

AU - Liu, Wangta

AU - Lin, Hao Yeh

AU - Chou, Hsin Yu

AU - Thia, Caroline

AU - Wu, Jou Hsien

AU - Chang, Jo Shu

AU - Wen, Zhi Hong

AU - Chang, Jui Jen

AU - David Wang, Hui Min

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N2 - 3S, 3′S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3′S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3′S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.

AB - 3S, 3′S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3′S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3′S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.

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