Metabolic reprogramming of hydrogenosomal amino acids in Trichomonas vaginalis under glucose restriction

Kuo Yang Huang, Seow Chin Ong, Chih Ching Wu, Chia Wei Hsu, Hsin Chung Lin, Yi Kai Fang, Wei Hung Cheng, Po Jung Huang, Cheng Hsun Chiu, Petrus Tang

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Background: Glucose is the major energy source that is converted to pyruvate for ATP generation in the trichomonad hydrogenosome. Under glucose restriction (GR), the regulation of amino acids metabolism is crucial for trichomonad growth and survival. RNA-sequencing (RNA-seq) analysis has been used to identify differentially expressed genes in Trichomonas vaginalis under GR, leading to significant advances in understanding adaptive responses of amino acid metabolism to GR. However, the levels of amino acid metabolites modulated by GR are unknown in T. vaginalis. Methods: Herein, we describe a comprehensive metabolomic analysis of amino acid metabolites in the hydrogenosome using liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry (LC-FT MS). The relative abundance of 17 hydrogenosomal amino acids was analyzed under GR and high-glucose (HG) conditions. Results: Levels of most amino acids were higher in GR culture. Arginine was not detectable in either HG or GR cultures; however, its metabolic end-product proline was slightly increased under GR, suggesting that the arginine dihydrolase pathway was more activated by GR. Additionally, methionine catabolism was less stimulated under GR because of greater methionine accumulation. Furthermore, branched chain amino acids (BCAA), including leucine, isoleucine and valine, as well as phenylalanine and alanine, markedly accumulated under GR, indicating that glutamate-related metabolic pathways were remarkably enhanced in this setting. Our metabolomic analysis combined with previous RNA-seq data confirm the existence of several amino acid metabolic pathways in the hydrogenosome and highlight their potentially important roles in T. vaginalis under glucose deprivation.

原文English
頁(從 - 到)630-637
頁數8
期刊Journal of Microbiology, Immunology and Infection
52
發行號4
DOIs
出版狀態Published - 2019 8月

All Science Journal Classification (ASJC) codes

  • 免疫學和過敏
  • 一般免疫學和微生物學
  • 微生物學(醫學)
  • 傳染性疾病

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