Metabolic substrates alter attachment and differentiated functions of proximal tubule cell culture

Ming Jer Tang, Richard L. Tannen

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

Proximal tubules cultured in vitro gradually lose their differentiated functions. Because standard culture media lacks several substrates important for renal proximal tubule oxidative metabolism, whether a mixture of substrates including butyrate, alanine, and lactate (BAL) would modify growth and/or differentiated function of proximal tubular cells in culture was examined. Tubules cultured in media supplemented with 2 mM butyrate, alanine, and lactate exhibited enhanced attachment but did not exhibit an altered growth rate. Higher levels of phosphoenolpyruvate carboxykinase and leucine-amino peptidase were sustained, although these activities were still diminished in comparison with that in fresh tubules. Sodium-dependent glucose uptake and dome formation - other reflections of epithelial cell differentiated function - also were enhanced. These studies demonstrate that the substrates used to culture proximal tubules can modify both their attachment and their manifestation of differentiated function in culture.

原文English
頁(從 - 到)1908-1911
頁數4
期刊Journal of the American Society of Nephrology
4
發行號11
出版狀態Published - 1994 五月

All Science Journal Classification (ASJC) codes

  • Nephrology

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