Metabolomics of a single vacuole reveals metabolic dynamism in an alga Chara australis

Akira Oikawa, Fumio Matsuda, Munehiro Kikuyama, Tetsuro Mimura, Kazuki Saito

研究成果: Article同行評審

52 引文 斯高帕斯(Scopus)

摘要

Metabolomics is the most reliable analytical method for understanding metabolic diversity in single organelles derived from single cells. Although metabolites such as phosphate compounds are believed to be localized in different organelles in a highly specific manner, the process of metabolite compartmentalization in the cell is not thoroughly understood. The analysis of metabolites in single organelles has consequently presented a significant challenge. In this study, we used a metabolomic method to elucidate the localization and dynamics of 125 known metabolites isolated from the vacuole and cytoplasm of a single cell of the alga Chara australis. The amount of metabolites in the vacuole and the cytoplasm fluctuated asynchronously under various stress conditions, suggesting that metabolites are spatially regulated within the cell. Metabolite transport across the vacuolar membrane can be directly detected using the microinjection technique, which may reveal a previously unknown function of the vacuole.

原文English
頁(從 - 到)544-551
頁數8
期刊Plant Physiology
157
發行號2
DOIs
出版狀態Published - 2011 10月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 遺傳學
  • 植物科學

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