Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells

I. Cheng Chou, Yee Lin Wu, Lin Chi Wang, Guo Ping Chang-Chien, Hsinyu Lee

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As5+, Cr6+, Cd2+, and Pb2+) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr6+ exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr6+ lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 μg-Cr/mL-medium (p < 0.05), respectively, but showed no significant difference at higher Cr6+concentrations (2.0-5.0 μg-Cr/mL-medium).

原文English
頁(從 - 到)881-885
頁數5
期刊Journal of Hazardous Materials
165
發行號1-3
DOIs
出版狀態Published - 2009 6月 15

All Science Journal Classification (ASJC) codes

  • 環境工程
  • 環境化學
  • 廢物管理和處置
  • 污染
  • 健康、毒理學和誘變

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