Metallo-β-lactamase-producing Enterobacteriaceae isolates in a university hospital in Taiwan: Prevalence of IMP-8 in Enterobacter cloacae and first identification of VIM-2 in Citrobacter freundii

Jing Jou Yan, Wen Chien Ko, Chin Luan Chuang, Jiunn Jong Wu

研究成果: Article同行評審

100 引文 斯高帕斯(Scopus)

摘要

A total of 9082 clinical isolates of Enterobacteriaceae other than Klebsiella spp. collected in 1999 and 2000 at a university hospital in Taiwan were investigated for the production of metallo-β-lactamases (MBLs). Thirty-six (2.9%) of the 1261 Enterobacter cloacae isolates and one (0.3%) of the 340 Citrobacter freundii isolates were found to carry blaIMP-8 and blaVIM-2, respectively, by colony hybridization, PCR and sequence analysis. The IMP-8 producers were recovered from 20 patients and four of them had recently transferred from other hospitals, implying spread of IMP-8-producing E. cloacae among different healthcare settings. Of the 20 non-repetitive IMP-8 producers, 17 (85%) isolates also harboured blaSHV-12, which was on the same transferable plasmids with blaIMP-8. The blaVIM-2-positive isolate and all non-repetitive blaIMP-8-positive isolates appeared susceptible to imipenem (MICs < 8 mg/L) and meropenem (MICs < 4 mg/L), indicating the difficulty in detection of MBLs in Enterobacteriaceae by routine susceptibility testing. Ribotyping of the IMP-8-producing E. cloacae isolates indicated that the dissemination of blaIMP-8 was due largely to the spread of an epidemic clone, but horizontal transfer among unrelated strains also occurred.

原文English
頁(從 - 到)503-511
頁數9
期刊Journal of Antimicrobial Chemotherapy
50
發行號4
出版狀態Published - 2002 十月 1

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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