Method and kit for detecting folate

貢獻的翻譯標題: 葉酸檢測方法及其檢測套組

Tzu-Fun Fu (Inventor)

研究成果: Patent

摘要

A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography.
原文English
專利號8663946
出版狀態Published - 2012 八月 16

指紋

Folic Acid
Catalysis
Centrifugation
High performance liquid chromatography
Hydrolases
Buffers
Cooling
Heating
Enzymes

引用此文

Fu, T-F. (2012). Method and kit for detecting folate. (專利號 8663946).
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Method and kit for detecting folate. / Fu, Tzu-Fun (Inventor).

專利號: 8663946.

研究成果: Patent

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AB - A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography.

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Fu T-F, 發明者. Method and kit for detecting folate. 8663946. 2012 8月 16.