Microcystin-LR biodegradation by bacillus sp. Reaction rates and possible genes involved in the degradation

Michelline M.R. Kansole, Tsair Fuh Lin

研究成果: Article同行評審

29 引文 斯高帕斯(Scopus)

摘要

Harmful cyanobacteria blooms may deteriorate freshwater environments, leading to bad water quality that can adversely affect the health of humans, animals, and aquatic life. Many cyanobacteria can produce toxic metabolites, with Microcystin-LR (MC-LR) being the most commonly detected cyanotoxin in fresh water bodies. In this study, a MC-LR degrading Bacillus sp. strain was isolated from Hulupi Lake (HLPL), Taiwan and tested for its degradability of the cyanotoxin. The results showed that the degradation of Microcystin-LR by the isolated Bacillus sp. was temperature-dependent with an optimum MC-LR removal at 37 °C and a first order degradation constant rate for 0.22 day-1. The degradation rate was also found to increase with decreasing MC-LR concentrations and increasing Bacillus sp. concentrations. Biomolecular monitoring of three types of genes (mlrA, CAAX, and GST) involved in the degradation indicated that mlrA, and CAAX genes were present in the indigenous bacteria in HLPL water samples. However, for the isolated Bacillus sp. strain, only CAAX genes were detected. The absence of the mlrA gene in the isolated Bacillus sp. strain shows that the degradation of MC-LR does not necessarily follow the pathways with mlrA, and can also follow the pathways involved with CAAX type II amino-terminal protease.

原文English
文章編號508
期刊Water (Switzerland)
8
發行號11
DOIs
出版狀態Published - 2016 11月 4

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 地理、規劃與發展
  • 海洋科學
  • 水科學與技術

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