Microfluidic chip of fast DNA hybridization using denature and motion of nucleic acids

Yung Chiang Chung, Yu-Cheng Lin, Chaung Di Chueh, Chuan You Ye, Li Wei Lai, Wei Chieh Liao

研究成果: Conference contribution

摘要

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region. Based on these results, the new hybridization chip with the processes of temperature and velocity differences will provide additional efficiency in DNA detection.

原文English
主出版物標題4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
頁面960-963
頁數4
DOIs
出版狀態Published - 2009 十月 12
事件4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
持續時間: 2009 一月 52009 一月 8

出版系列

名字4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

Other

Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
國家/地區China
城市Shenzhen
期間09-01-0509-01-08

All Science Journal Classification (ASJC) codes

  • 電氣與電子工程

指紋

深入研究「Microfluidic chip of fast DNA hybridization using denature and motion of nucleic acids」主題。共同形成了獨特的指紋。

引用此