Microfluidic chip of fast DNA hybridization using denature and motion of nucleic acids

Yung Chiang Chung, Yu Cheng Lin, Chaung Di Chueh, Chuan You Ye, Li Wei Lai

研究成果: Paper同行評審

摘要

We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb target DNA as the test material. The increase in fluorescence intensity was apparent when the temperature was higher than 82°C, and the fluorescence intensity reached an asymptotic value as T > 90°C. A mathematical model was proposed to relate the fluorescence intensity of DNA hybridization with the temperature of hot region and the velocity of cold region.

原文English
頁面1675-1677
頁數3
出版狀態Published - 2008
事件12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
持續時間: 2008 10月 122008 10月 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
國家/地區United States
城市San Diego, CA
期間08-10-1208-10-16

All Science Journal Classification (ASJC) codes

  • 化學工程(雜項)
  • 生物工程

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