Microfluidic paper-based platform for whole blood creatinine detection

研究成果: Article

16 引文 (Scopus)

摘要

An integrated platform consisting of a three-dimensional (3-D) microfluidic paper-based analytical device (µPAD) and a portable detection system is proposed for the determination of human whole blood creatinine. In the proposed detection method, the reaction region of the 3-D µPAD is implanted with picric acid and NaOH reagent and allowed to dry. Whole human blood is then dripped onto the inlet region of the chip and the blood plasma diffuses through the separation channel into the reaction zone under the effects of capillary action. The µPAD is heated at a temperature of 37 °C for 5 min to induce a Jaffé reaction between the creatinine in the blood plasma and the reagent. Finally, the creatinine concentration is detected using a colorimetric method. The validity of the proposed approach is demonstrated using control samples with creatinine concentrations ranging from 0.19 to 7.64 mg/dL. The detection results obtained for 40 real-world human serum creatinine samples and 30 real-world whole blood samples are shown to be in excellent agreement with those obtained using a conventional macroscale assay technique. Overall, the results show that the proposed platform provides a compact, low-cost and reliable approach for whole blood creatinine concentration detection.

原文English
頁(從 - 到)117-124
頁數8
期刊Chemical Engineering Journal
348
DOIs
出版狀態Published - 2018 九月 15

指紋

Microfluidics
Creatinine
Blood
blood
Plasmas
plasma
detection method
paper
detection
serum
Assays
assay
Acids
acid
cost
Costs
temperature
Temperature

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

引用此文

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title = "Microfluidic paper-based platform for whole blood creatinine detection",
abstract = "An integrated platform consisting of a three-dimensional (3-D) microfluidic paper-based analytical device (µPAD) and a portable detection system is proposed for the determination of human whole blood creatinine. In the proposed detection method, the reaction region of the 3-D µPAD is implanted with picric acid and NaOH reagent and allowed to dry. Whole human blood is then dripped onto the inlet region of the chip and the blood plasma diffuses through the separation channel into the reaction zone under the effects of capillary action. The µPAD is heated at a temperature of 37 °C for 5 min to induce a Jaff{\'e} reaction between the creatinine in the blood plasma and the reagent. Finally, the creatinine concentration is detected using a colorimetric method. The validity of the proposed approach is demonstrated using control samples with creatinine concentrations ranging from 0.19 to 7.64 mg/dL. The detection results obtained for 40 real-world human serum creatinine samples and 30 real-world whole blood samples are shown to be in excellent agreement with those obtained using a conventional macroscale assay technique. Overall, the results show that the proposed platform provides a compact, low-cost and reliable approach for whole blood creatinine concentration detection.",
author = "Tseng, {Chin Chung} and Yang, {Ruey Jen} and Ju, {Wei Jhong} and Fu, {Lung Ming}",
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AU - Yang, Ruey Jen

AU - Ju, Wei Jhong

AU - Fu, Lung Ming

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Y1 - 2018/9/15

N2 - An integrated platform consisting of a three-dimensional (3-D) microfluidic paper-based analytical device (µPAD) and a portable detection system is proposed for the determination of human whole blood creatinine. In the proposed detection method, the reaction region of the 3-D µPAD is implanted with picric acid and NaOH reagent and allowed to dry. Whole human blood is then dripped onto the inlet region of the chip and the blood plasma diffuses through the separation channel into the reaction zone under the effects of capillary action. The µPAD is heated at a temperature of 37 °C for 5 min to induce a Jaffé reaction between the creatinine in the blood plasma and the reagent. Finally, the creatinine concentration is detected using a colorimetric method. The validity of the proposed approach is demonstrated using control samples with creatinine concentrations ranging from 0.19 to 7.64 mg/dL. The detection results obtained for 40 real-world human serum creatinine samples and 30 real-world whole blood samples are shown to be in excellent agreement with those obtained using a conventional macroscale assay technique. Overall, the results show that the proposed platform provides a compact, low-cost and reliable approach for whole blood creatinine concentration detection.

AB - An integrated platform consisting of a three-dimensional (3-D) microfluidic paper-based analytical device (µPAD) and a portable detection system is proposed for the determination of human whole blood creatinine. In the proposed detection method, the reaction region of the 3-D µPAD is implanted with picric acid and NaOH reagent and allowed to dry. Whole human blood is then dripped onto the inlet region of the chip and the blood plasma diffuses through the separation channel into the reaction zone under the effects of capillary action. The µPAD is heated at a temperature of 37 °C for 5 min to induce a Jaffé reaction between the creatinine in the blood plasma and the reagent. Finally, the creatinine concentration is detected using a colorimetric method. The validity of the proposed approach is demonstrated using control samples with creatinine concentrations ranging from 0.19 to 7.64 mg/dL. The detection results obtained for 40 real-world human serum creatinine samples and 30 real-world whole blood samples are shown to be in excellent agreement with those obtained using a conventional macroscale assay technique. Overall, the results show that the proposed platform provides a compact, low-cost and reliable approach for whole blood creatinine concentration detection.

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