Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

Lien Yu Hung, Chien Yu Fu, Chih Hung Wang, Yuan Jhe Chuang, Yi Cheng Tsai, Yi Ling Lo, Wen Bin Lee, Shu-Chu Shiesh, Hwan You Chang, Keng-Fu Hsu, Gwo Bin Lee

研究成果: Conference contribution

2 引文 (Scopus)

摘要

This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

原文English
主出版物標題TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
發行者Institute of Electrical and Electronics Engineers Inc.
頁面568-571
頁數4
ISBN(電子)9781538627310
DOIs
出版狀態Published - 2017 七月 26
事件19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan
持續時間: 2017 六月 182017 六月 22

出版系列

名字TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
國家Taiwan
城市Kaohsiung
期間17-06-1817-06-22

指紋

Bacteriophages
chutes
Microfluidics
reagents
affinity
Screening
screening
platforms
Biopsy
cancer
Display devices
Tissue
Peptides
peptides
Peptide Aptamers
Peptide Nucleic Acids
Nucleic acids
nucleic acids
amino acids
Amino acids

All Science Journal Classification (ASJC) codes

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

引用此文

Hung, L. Y., Fu, C. Y., Wang, C. H., Chuang, Y. J., Tsai, Y. C., Lo, Y. L., ... Lee, G. B. (2017). Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. 於 TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (頁 568-571). [7994112] (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994112
Hung, Lien Yu ; Fu, Chien Yu ; Wang, Chih Hung ; Chuang, Yuan Jhe ; Tsai, Yi Cheng ; Lo, Yi Ling ; Lee, Wen Bin ; Shiesh, Shu-Chu ; Chang, Hwan You ; Hsu, Keng-Fu ; Lee, Gwo Bin. / Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. 頁 568-571 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).
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title = "Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer",
abstract = "This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.",
author = "Hung, {Lien Yu} and Fu, {Chien Yu} and Wang, {Chih Hung} and Chuang, {Yuan Jhe} and Tsai, {Yi Cheng} and Lo, {Yi Ling} and Lee, {Wen Bin} and Shu-Chu Shiesh and Chang, {Hwan You} and Keng-Fu Hsu and Lee, {Gwo Bin}",
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Hung, LY, Fu, CY, Wang, CH, Chuang, YJ, Tsai, YC, Lo, YL, Lee, WB, Shiesh, S-C, Chang, HY, Hsu, K-F & Lee, GB 2017, Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. 於 TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994112, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., 頁 568-571, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, 17-06-18. https://doi.org/10.1109/TRANSDUCERS.2017.7994112

Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. / Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung; Chuang, Yuan Jhe; Tsai, Yi Cheng; Lo, Yi Ling; Lee, Wen Bin; Shiesh, Shu-Chu; Chang, Hwan You; Hsu, Keng-Fu; Lee, Gwo Bin.

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 568-571 7994112 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

研究成果: Conference contribution

TY - GEN

T1 - Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

AU - Hung, Lien Yu

AU - Fu, Chien Yu

AU - Wang, Chih Hung

AU - Chuang, Yuan Jhe

AU - Tsai, Yi Cheng

AU - Lo, Yi Ling

AU - Lee, Wen Bin

AU - Shiesh, Shu-Chu

AU - Chang, Hwan You

AU - Hsu, Keng-Fu

AU - Lee, Gwo Bin

PY - 2017/7/26

Y1 - 2017/7/26

N2 - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

AB - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

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U2 - 10.1109/TRANSDUCERS.2017.7994112

DO - 10.1109/TRANSDUCERS.2017.7994112

M3 - Conference contribution

AN - SCOPUS:85029361482

T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

SP - 568

EP - 571

BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

PB - Institute of Electrical and Electronics Engineers Inc.

ER -

Hung LY, Fu CY, Wang CH, Chuang YJ, Tsai YC, Lo YL 等. Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. 於 TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 568-571. 7994112. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/TRANSDUCERS.2017.7994112