摘要
This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.
| 原文 | English |
|---|---|
| 主出版物標題 | TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems |
| 發行者 | Institute of Electrical and Electronics Engineers Inc. |
| 頁面 | 568-571 |
| 頁數 | 4 |
| ISBN（電子） | 9781538627310 |
| DOIs | |
| 出版狀態 | Published - 2017 七月 26 |
| 事件 | 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan 持續時間: 2017 六月 18 → 2017 六月 22 |
出版系列
| 名字 | TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems |
|---|
Other
| Other | 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 |
|---|---|
| 國家 | Taiwan |
| 城市 | Kaohsiung |
| 期間 | 17-06-18 → 17-06-22 |
指紋
All Science Journal Classification (ASJC) codes
- Chemical Health and Safety
- Instrumentation
- Electrical and Electronic Engineering
引用此文
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Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. / Hung, Lien Yu; Fu, Chien Yu; Wang, Chih Hung; Chuang, Yuan Jhe; Tsai, Yi Cheng; Lo, Yi Ling; Lee, Wen Bin; Shiesh, Shu-Chu; Chang, Hwan You; Hsu, Keng-Fu; Lee, Gwo Bin.
TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 568-571 7994112 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).研究成果: Conference contribution
TY - GEN
T1 - Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer
AU - Hung, Lien Yu
AU - Fu, Chien Yu
AU - Wang, Chih Hung
AU - Chuang, Yuan Jhe
AU - Tsai, Yi Cheng
AU - Lo, Yi Ling
AU - Lee, Wen Bin
AU - Shiesh, Shu-Chu
AU - Chang, Hwan You
AU - Hsu, Keng-Fu
AU - Lee, Gwo Bin
PY - 2017/7/26
Y1 - 2017/7/26
N2 - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.
AB - This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.
UR - http://www.scopus.com/inward/record.url?scp=85029361482&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029361482&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2017.7994112
DO - 10.1109/TRANSDUCERS.2017.7994112
M3 - Conference contribution
AN - SCOPUS:85029361482
T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 568
EP - 571
BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PB - Institute of Electrical and Electronics Engineers Inc.
ER -