Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer

Lien Yu Hung, Chien Yu Fu, Chih Hung Wang, Yuan Jhe Chuang, Yi Cheng Tsai, Yi Ling Lo, Wen Bin Lee, Shu Chu Shiesh, Hwan You Chang, Keng Fu Hsu, Gwo Bin Lee

研究成果: Conference contribution

2 引文 斯高帕斯(Scopus)

摘要

This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that a solid biopsy (tissue slide) was used on an integrated microfluidic system for screening of two kinds of affinity reagents, including aptamers (nucleic acids type) and peptides (amino acids type). The screened aptamers and peptides have been tested with high affinity and specificity toward target cancer.

原文English
主出版物標題TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
發行者Institute of Electrical and Electronics Engineers Inc.
頁面568-571
頁數4
ISBN(電子)9781538627310
DOIs
出版狀態Published - 2017 七月 26
事件19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan
持續時間: 2017 六月 182017 六月 22

出版系列

名字TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
國家Taiwan
城市Kaohsiung
期間17-06-1817-06-22

    指紋

All Science Journal Classification (ASJC) codes

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

引用此

Hung, L. Y., Fu, C. Y., Wang, C. H., Chuang, Y. J., Tsai, Y. C., Lo, Y. L., Lee, W. B., Shiesh, S. C., Chang, H. Y., Hsu, K. F., & Lee, G. B. (2017). Microfluidic platform capable of performing automatic tissue slide-based selex and phage display for rapid screening of affinity reagents specific to ovarian cancer. 於 TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (頁 568-571). [7994112] (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994112