TY - JOUR
T1 - MicroRNA-133 suppresses cell viability and migration of rheumatoid arthritis fibroblast-like synoviocytes by down-regulation of MET, EGFR, and FSCN1 expression
AU - Chen, Shih Yao
AU - Hsieh, Jeng Long
AU - Wu, Po Ting
AU - Shiau, Ai Li
AU - Wu, Chao Liang
N1 - Funding Information:
We thank Dr. D. Trono (Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland) for generously providing psPAX2 and pMD2.G plasmids for the production of lentiviral vectors and the Laboratory Animal Center, College of Medicine, National Cheng Kung University and Taiwan Animal Consortium (AAALAC International Full Accreditation) for the animal care. We also thank professor Chi-Chien Lin helps us collect synovial tissue samples at Taichung Veterans General Hospital, Taichung, Taiwan.
Funding Information:
This work was supported by MOST 108-2314-B-006-037-MY3, and 108-2314-B-273-005-MY3 from the Ministry of Science and Technology, Taiwan.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/11
Y1 - 2022/11
N2 - Aberrant proliferation and migration of fibroblast-like synoviocytes (FLS) are major characteristics of rheumatoid arthritis (RA). MicroRNA-133 (miR-133) is a tumor-suppressive miRNA that targets various genes responsive for cell proliferation and migration. The aim of this study was to examine the effect of miR-133 on RA FLS. A high throughput miRNA microarray was performed in synovium from mice with collagen-induced arthritis (CIA). Expression levels of miR-133 and the putative targets were determined in synovium and FLS from patients with RA and mice with CIA. Overexpression of miR-133 in RA FLS was performed by lentiviral vector-mediated transfer of precursor miRNA (pre-miR). The expression of miR-133a/b was decreased in the joint tissue and FLS of CIA mice, as determined by miRNA array and qRT-PCR. Down-regulation of miR-133a/b expression could also be observed in synovium and FLS from patients with RA. Overexpression of miR-133 reduced cell viability and migration of RA FLS, with decreased levels of FSCN1, EGFR, and MET. Our findings demonstrated the inhibitory effects of miR-133 on FLS viability and migration, and might contribute to the pharmacologic development of miR-133 therapeutics in patients with RA.
AB - Aberrant proliferation and migration of fibroblast-like synoviocytes (FLS) are major characteristics of rheumatoid arthritis (RA). MicroRNA-133 (miR-133) is a tumor-suppressive miRNA that targets various genes responsive for cell proliferation and migration. The aim of this study was to examine the effect of miR-133 on RA FLS. A high throughput miRNA microarray was performed in synovium from mice with collagen-induced arthritis (CIA). Expression levels of miR-133 and the putative targets were determined in synovium and FLS from patients with RA and mice with CIA. Overexpression of miR-133 in RA FLS was performed by lentiviral vector-mediated transfer of precursor miRNA (pre-miR). The expression of miR-133a/b was decreased in the joint tissue and FLS of CIA mice, as determined by miRNA array and qRT-PCR. Down-regulation of miR-133a/b expression could also be observed in synovium and FLS from patients with RA. Overexpression of miR-133 reduced cell viability and migration of RA FLS, with decreased levels of FSCN1, EGFR, and MET. Our findings demonstrated the inhibitory effects of miR-133 on FLS viability and migration, and might contribute to the pharmacologic development of miR-133 therapeutics in patients with RA.
UR - https://www.scopus.com/pages/publications/85130235783
UR - https://www.scopus.com/pages/publications/85130235783#tab=citedBy
U2 - 10.1007/s11010-022-04457-6
DO - 10.1007/s11010-022-04457-6
M3 - Article
C2 - 35595956
AN - SCOPUS:85130235783
SN - 0300-8177
VL - 477
SP - 2529
EP - 2537
JO - Molecular and Cellular Biochemistry
JF - Molecular and Cellular Biochemistry
IS - 11
ER -