Microwell-assisted filtration with anodic aluminum oxide membrane for Raman analysis of algal cells

Fang Yu Wen, Po Sheng Chen, Ting Wei Liao, Yi Je Juang

研究成果: Article

1 引文 (Scopus)

摘要

In this study, rapid and direct Raman analysis of microalgae was demonstrated through measurement of algal cells (or algal paste) prepared by microwell-assisted filtration using an anodic aluminum oxide (AAO) membrane. To obtain the algal paste, an array of polydimethylsiloxane (PDMS) microwells with through holes was placed on top of the AAO membrane, after which a vacuum was initiated and a droplet of algal solution dispensed inside the PDMS microwells for a 5-min filtration process. The results showed that the AAO membrane has the lowest background noise compared to other types of filter papers or membranes. Compared to the droplet evaporation method, the “coffee-ring” effect was effectively suppressed when applying our proposed filtration method. The algal cells became stacked inside the microwells, which led to relatively uniform and strong Raman signals across individual algal paste and consistency among different algal pastes.

原文English
頁(從 - 到)412-418
頁數7
期刊Algal Research
33
DOIs
出版狀態Published - 2018 七月

指紋

aluminum oxide
droplets
cells
microalgae
evaporation
methodology

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

引用此文

Wen, Fang Yu ; Chen, Po Sheng ; Liao, Ting Wei ; Juang, Yi Je. / Microwell-assisted filtration with anodic aluminum oxide membrane for Raman analysis of algal cells. 於: Algal Research. 2018 ; 卷 33. 頁 412-418.
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abstract = "In this study, rapid and direct Raman analysis of microalgae was demonstrated through measurement of algal cells (or algal paste) prepared by microwell-assisted filtration using an anodic aluminum oxide (AAO) membrane. To obtain the algal paste, an array of polydimethylsiloxane (PDMS) microwells with through holes was placed on top of the AAO membrane, after which a vacuum was initiated and a droplet of algal solution dispensed inside the PDMS microwells for a 5-min filtration process. The results showed that the AAO membrane has the lowest background noise compared to other types of filter papers or membranes. Compared to the droplet evaporation method, the “coffee-ring” effect was effectively suppressed when applying our proposed filtration method. The algal cells became stacked inside the microwells, which led to relatively uniform and strong Raman signals across individual algal paste and consistency among different algal pastes.",
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Microwell-assisted filtration with anodic aluminum oxide membrane for Raman analysis of algal cells. / Wen, Fang Yu; Chen, Po Sheng; Liao, Ting Wei; Juang, Yi Je.

於: Algal Research, 卷 33, 07.2018, p. 412-418.

研究成果: Article

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