Mini chamber system for long-term maintenance and observation of cultured cells

Chung Liang Ho, Tun Yi Mou, Pei Shuan Chiang, Chu Li Weng, Nan Haw Chow

研究成果: Article同行評審

23 引文 斯高帕斯(Scopus)

摘要

We constructed a mini chamber system that was able to maintain cell culture on a microscope for long periods. It is a modified closed system with medium perfusion and CO2 circulation. The closed CO2 circulation and ample air inside the chamber distinguish it from other closed systems. Using different cell lines, the system was shown to be able to support long-term, time-lapse recording. After 229 hours of time-lapse recording, A2058 cells (a melanoma cell line) became overconfluent but still multiplied. Many CAD cells (a marine neuron-like cell line) still moved their cell bodies and kept their neurite-like processes after 28 days of recording. The entire healing process of a scratch-wounded T24 (a bladder cancer cell line) monolayer can be monitored. Such a modified closed system should find many applications in developmental biology, cell biology, and cancer biology where long-term, time-lapse recording is required or when the health of cells is important.

原文English
頁(從 - 到)267-273
頁數7
期刊BioTechniques
38
發行號2
DOIs
出版狀態Published - 2005 二月

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物化學、遺傳與分子生物學 (全部)

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