Modification of activation kinetics of delayed rectifier K+ currents and neuronal excitability by methyl-β-cyclodextrin

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

The effects of methyl-β-cyclodextrin (MβCD), an oligosaccharide, on ion currents were investigated in differentiated NG108-15 neuronal cells. In NG108-15 cells treated with dibutyryl cyclic AMP, the expression level of the KV3.1 mRNA was elevated. Depletion of membrane cholesterol by exposing cells to MβCD (1 mM) resulted in a significant reduction of the activation kinetics of delayed rectifier K+ current (IK(DR)) in these cells. However, neither activation nor inactivation curve of IK(DR) was altered following MβCD treatment. In current-clamp recordings, in MβCD-treated cells, the instantaneous frequency of the firing in response to long-lasting current stimuli was reduced. In a modified Hodgkin-Huxley neuron, the upward shift in the relationship of activation/deactivation time constant of IK(DR) versus membrane potential causes a reduction of IK(DR) amplitude accompanied by an increase in the width of action potentials. In the studies from a high-frequency modeled neuron, reduction of voltage-dependent activation of IK(DR) can also facilitate spike-frequency adaptation. In a simulated network of spiking neurons, the increased activation/deactivation time constant of IK(DR) slowed repetitive firing. Taken together, MβCD may slow activation kinetics of IK(DR) and confer a trigger for the propensity to develop spike-frequency adaptation in neurons or neuroendocrine cells.

原文English
頁(從 - 到)431-441
頁數11
期刊Neuroscience
176
DOIs
出版狀態Published - 2011 3月 10

All Science Journal Classification (ASJC) codes

  • 一般神經科學

指紋

深入研究「Modification of activation kinetics of delayed rectifier K+ currents and neuronal excitability by methyl-β-cyclodextrin」主題。共同形成了獨特的指紋。

引用此