Modularly assembled magnetite nanoparticles enhance in vivo targeting for magnetic resonance cancer imaging

Ping Ching Wu, Chia Hao Su, Fong Yu Cheng, Jun Cheng Weng, Jyh Horng Chen, Tsung Lin Tsai, Chen Sheng Yeh, Wu Chou Su, Jih Ru Hwu, Yonhua Tzeng, Dar Bin Shieh

研究成果: Article

40 引文 (Scopus)

摘要

Modularly assembled targeting nanoparticles were synthesized through self-assembly of targeting moieties on surfaces of functional nanoparticles. Specific molecular recognition of nickel nitrilotriacetate on Fe 3O4 nanoparticles with hexahistidine tag on RGD4C peptides results in precisely controlled orientation of the targeting peptides. Better selectivity of the self-assembled RGD4C-Fe3O4 nanoparticles targeting oral cancer cells than that achievable through a conventional chemical cross-link strategy was demonstrated by means of atomic absorption spectrometry (AAS). An oral cancer hamster model was applied to reveal specific in vivo targeting and MR molecular imaging contrast in cancer lesions expressing αvβ3 integrin. Both AAS and MRI revealed that the self-assembled nanoparticles improved the targeting efficiency and reduced the hepatic uptake as compared with the conventional chemical cross-link particles. We investigated the biosafety, biodistribution, and kinetics of the nanoparticles and found that the nanoparticles were significantly cleared from the liver and kidneys after one week. By recombining the desired targeting moiety and various functional nanoparticles through self-assembly, this new modularly designed platform has the capability of enhancing the efficiency of targeted diagnosis and therapies for a wide spectrum of biomedical applications.

原文English
頁(從 - 到)1972-1979
頁數8
期刊Bioconjugate Chemistry
19
發行號10
DOIs
出版狀態Published - 2008 十月 1

指紋

Magnetite Nanoparticles
Magnetite nanoparticles
Magnetic resonance
Nanoparticles
Magnetic Resonance Imaging
Imaging techniques
Neoplasms
Atomic absorption spectrometry
His-His-His-His-His-His
Mouth Neoplasms
Self assembly
Peptides
Spectrum Analysis
Molecular imaging
Molecular recognition
Molecular Imaging
Liver
Nickel
Integrins
Cricetinae

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

引用此文

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title = "Modularly assembled magnetite nanoparticles enhance in vivo targeting for magnetic resonance cancer imaging",
abstract = "Modularly assembled targeting nanoparticles were synthesized through self-assembly of targeting moieties on surfaces of functional nanoparticles. Specific molecular recognition of nickel nitrilotriacetate on Fe 3O4 nanoparticles with hexahistidine tag on RGD4C peptides results in precisely controlled orientation of the targeting peptides. Better selectivity of the self-assembled RGD4C-Fe3O4 nanoparticles targeting oral cancer cells than that achievable through a conventional chemical cross-link strategy was demonstrated by means of atomic absorption spectrometry (AAS). An oral cancer hamster model was applied to reveal specific in vivo targeting and MR molecular imaging contrast in cancer lesions expressing αvβ3 integrin. Both AAS and MRI revealed that the self-assembled nanoparticles improved the targeting efficiency and reduced the hepatic uptake as compared with the conventional chemical cross-link particles. We investigated the biosafety, biodistribution, and kinetics of the nanoparticles and found that the nanoparticles were significantly cleared from the liver and kidneys after one week. By recombining the desired targeting moiety and various functional nanoparticles through self-assembly, this new modularly designed platform has the capability of enhancing the efficiency of targeted diagnosis and therapies for a wide spectrum of biomedical applications.",
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Modularly assembled magnetite nanoparticles enhance in vivo targeting for magnetic resonance cancer imaging. / Wu, Ping Ching; Su, Chia Hao; Cheng, Fong Yu; Weng, Jun Cheng; Chen, Jyh Horng; Tsai, Tsung Lin; Yeh, Chen Sheng; Su, Wu Chou; Hwu, Jih Ru; Tzeng, Yonhua; Shieh, Dar Bin.

於: Bioconjugate Chemistry, 卷 19, 編號 10, 01.10.2008, p. 1972-1979.

研究成果: Article

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AU - Tsai, Tsung Lin

AU - Yeh, Chen Sheng

AU - Su, Wu Chou

AU - Hwu, Jih Ru

AU - Tzeng, Yonhua

AU - Shieh, Dar Bin

PY - 2008/10/1

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N2 - Modularly assembled targeting nanoparticles were synthesized through self-assembly of targeting moieties on surfaces of functional nanoparticles. Specific molecular recognition of nickel nitrilotriacetate on Fe 3O4 nanoparticles with hexahistidine tag on RGD4C peptides results in precisely controlled orientation of the targeting peptides. Better selectivity of the self-assembled RGD4C-Fe3O4 nanoparticles targeting oral cancer cells than that achievable through a conventional chemical cross-link strategy was demonstrated by means of atomic absorption spectrometry (AAS). An oral cancer hamster model was applied to reveal specific in vivo targeting and MR molecular imaging contrast in cancer lesions expressing αvβ3 integrin. Both AAS and MRI revealed that the self-assembled nanoparticles improved the targeting efficiency and reduced the hepatic uptake as compared with the conventional chemical cross-link particles. We investigated the biosafety, biodistribution, and kinetics of the nanoparticles and found that the nanoparticles were significantly cleared from the liver and kidneys after one week. By recombining the desired targeting moiety and various functional nanoparticles through self-assembly, this new modularly designed platform has the capability of enhancing the efficiency of targeted diagnosis and therapies for a wide spectrum of biomedical applications.

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