Molecular cloning, characterization and tissue expression of prophenoloxidase cDNA from the mosquito Armigeres subalbatus inoculated with Dirofilaria immitis microfilariae

W. L. Cho, H. S. Liu, C. H. Lee, C. C. Kuo, T. Y. Chang, C. T. Liu, C. C. Chen

研究成果: Article同行評審

52 引文 斯高帕斯(Scopus)

摘要

A cDNA encoding mosquito Armigeres subalbatus prophenol oxidase (As-pro-PO) was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) after Dirofliaria immitis inoculation. The 2205 bp As-pro-PO cDNA contains a 32 bp 5'-noncoding region, a 2055 bp open reading frame (685 amino acids), and a 118 bp 3'-noncoding region. Hydrophobic signal peptide for the endoplasmic reticulum targeting is not found in the NN2-terminal region. Two potential copper-binding domains, amino acids 197-245 and 345-412, are highly homologous to those of the other insect pro-POs. A 2.2 kb As-pro-PO transcript was identified by Northern blot analysis using D. immitis microfilariae-inoculated A. subalbatus. Both in situ hybridization and Northern blot analysis demonstrated that As-pro-PO mRNA was synthesized in mosquito haemocytes but not in other tissues, i.e. fat bodies, midguts and ovaries, etc.

原文English
頁(從 - 到)31-40
頁數10
期刊Insect Molecular Biology
7
發行號1
DOIs
出版狀態Published - 1998 2月

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 遺傳學
  • 昆蟲科學

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