Molecular detection and comparison of Acanthamoeba genotypes in different functions of watersheds in Taiwan

Po Min Kao, Bing Mu Hsu, Nai Hsiung Chen, Kuan Hao Huang, Chin Chun Huang, Dar Der Ji, Jung Sheng Chen, Wei-Chen Lin, Shih Wei Huang, Yi Chou Chiu

研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)

摘要

The protistan genus Acanthamoeba is a free-living amoeba existing in various environments. Within this protistan genus, there are some species recognized as potential human pathogens, which may cause granulomatous amoebic encephalitis, Acantha-moeba keratitis and chronic granulomatous lesions of the skin. In this study, 211 water samples were collected from two watersheds in southern Taiwan. We detected Acanthamoeba based on the PCR amplification with a genus-specific primer pair and investigation of Acanthamoeba in Puzih River and Kaoping River in southern Taiwan. Acanthamoeba species were detected in 34 (16.1%) samples. The presence or absence of Acanthamoeba within the water samples showed significant difference with the levels of water temperature and total coliforms. The most frequently identified Acanthamoeba genotype was T4 (n=19), followed by T5 (n=8), and then T15 (n=3). Genotype T6, T7/T8, T11 and T12 were all detected once. Genotype T4, T5, T6, T11 and T15 of Acanthamoeba are responsible for Acanthamoeba keratitis and should be considered a potential health threat associated with human activities in environmental surface water watersheds.

原文English
頁(從 - 到)4335-4344
頁數10
期刊Environmental Monitoring and Assessment
184
發行號7
DOIs
出版狀態Published - 2012 七月 1

All Science Journal Classification (ASJC) codes

  • Environmental Science(all)
  • Pollution
  • Management, Monitoring, Policy and Law

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