Molecular diagnostic markers for lung cancer in sputum and plasma

Yi Ching Wang, Han Shui Hsu, Tsz Pei Chen, Jung Ta Chen

研究成果: Conference contribution

46 引文 (Scopus)

摘要

Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation-specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.

原文English
主出版物標題Circulating Nucleic Acids in Plasma and Serum IV
發行者Blackwell Publishing Inc.
頁面179-184
頁數6
ISBN(列印)157331627X, 9781573316279
DOIs
出版狀態Published - 2006 九月

出版系列

名字Annals of the New York Academy of Sciences
1075
ISSN(列印)0077-8923
ISSN(電子)1749-6632

指紋

Molecular Pathology
Biomarkers
Sputum
Lung Neoplasms
Microsatellite Repeats
Plasmas
Microsatellite Instability
Methylation
Loss of Heterozygosity
Tumors
Sensitivity and Specificity
Tumor Suppressor Genes
Genetic Markers
Early Detection of Cancer
Cause of Death
Neoplasms
Genes
Tissue
Polymerase Chain Reaction
Lung

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

引用此文

Wang, Y. C., Hsu, H. S., Chen, T. P., & Chen, J. T. (2006). Molecular diagnostic markers for lung cancer in sputum and plasma. 於 Circulating Nucleic Acids in Plasma and Serum IV (頁 179-184). (Annals of the New York Academy of Sciences; 卷 1075). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1368.024
Wang, Yi Ching ; Hsu, Han Shui ; Chen, Tsz Pei ; Chen, Jung Ta. / Molecular diagnostic markers for lung cancer in sputum and plasma. Circulating Nucleic Acids in Plasma and Serum IV. Blackwell Publishing Inc., 2006. 頁 179-184 (Annals of the New York Academy of Sciences).
@inproceedings{359ef1000a0c401f837119f213640903,
title = "Molecular diagnostic markers for lung cancer in sputum and plasma",
abstract = "Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation-specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.",
author = "Wang, {Yi Ching} and Hsu, {Han Shui} and Chen, {Tsz Pei} and Chen, {Jung Ta}",
year = "2006",
month = "9",
doi = "10.1196/annals.1368.024",
language = "English",
isbn = "157331627X",
series = "Annals of the New York Academy of Sciences",
publisher = "Blackwell Publishing Inc.",
pages = "179--184",
booktitle = "Circulating Nucleic Acids in Plasma and Serum IV",

}

Wang, YC, Hsu, HS, Chen, TP & Chen, JT 2006, Molecular diagnostic markers for lung cancer in sputum and plasma. 於 Circulating Nucleic Acids in Plasma and Serum IV. Annals of the New York Academy of Sciences, 卷 1075, Blackwell Publishing Inc., 頁 179-184. https://doi.org/10.1196/annals.1368.024

Molecular diagnostic markers for lung cancer in sputum and plasma. / Wang, Yi Ching; Hsu, Han Shui; Chen, Tsz Pei; Chen, Jung Ta.

Circulating Nucleic Acids in Plasma and Serum IV. Blackwell Publishing Inc., 2006. p. 179-184 (Annals of the New York Academy of Sciences; 卷 1075).

研究成果: Conference contribution

TY - GEN

T1 - Molecular diagnostic markers for lung cancer in sputum and plasma

AU - Wang, Yi Ching

AU - Hsu, Han Shui

AU - Chen, Tsz Pei

AU - Chen, Jung Ta

PY - 2006/9

Y1 - 2006/9

N2 - Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation-specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.

AB - Lung cancer is the leading cause of cancer deaths worldwide. This study was designed to select multiple DNA markers, which have high sensitivity and specificity to serve as biomarkers for diagnosis of lung cancer. We examined the promoter hypermethylation of three tumor suppressor genes by methylation-specific PCR (MSP), and the instability of eight microsatellite markers by loss of heterozygosity (LOH) and microsatellite instability (MSI) analyses in lung tumor tissues and matched sputum specimens from 79 lung cancer patients. On the basis of the results of sensitivity, specificity, and concordance from each marker analyzed, we selected seven biomarkers, which are LOH of D9S286, D9S942, GATA49D12, and D13S170, MSI of D9S942, and methylation of p16INK4a and RARβ, from the sputum analyses. These selected etiologically associated biomarkers can potentially be used as supplemental diagnostic biomarkers for early lung cancer detection.

UR - http://www.scopus.com/inward/record.url?scp=33749582789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749582789&partnerID=8YFLogxK

U2 - 10.1196/annals.1368.024

DO - 10.1196/annals.1368.024

M3 - Conference contribution

C2 - 17108209

AN - SCOPUS:33749582789

SN - 157331627X

SN - 9781573316279

T3 - Annals of the New York Academy of Sciences

SP - 179

EP - 184

BT - Circulating Nucleic Acids in Plasma and Serum IV

PB - Blackwell Publishing Inc.

ER -

Wang YC, Hsu HS, Chen TP, Chen JT. Molecular diagnostic markers for lung cancer in sputum and plasma. 於 Circulating Nucleic Acids in Plasma and Serum IV. Blackwell Publishing Inc. 2006. p. 179-184. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1368.024