Molecularly imprinted electrochemical sensing of urinary melatonin in a microfluidic system

Mei Hwa Lee, Danny O'Hare, Yi Li Chen, Yu Chia Chang, Chien Hsin Yang, Bin Da Liu, Hung Yin Lin

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Melatonin levels may be related to the risks of breast cancer and prostate cancer. The measurement of urinary melatonin is also useful in monitoring serum melatonin levels following oral administration. In this work, melatonin is the target molecule, which is imprinted onto poly(ethylene-co-vinyl alcohol) by evaporation of the solvent on the working electrode of an electrochemical sensing chip. This sensing chip is used directly as a tool for optimizing the imprinting polymer composition, flow rate, and injection volume of the samples. Microfluidic sensing of the target and interference molecules revealed that the lowest detection limit is as low as ~pM, and the electrochemical response is weak even at high interference concentrations. Poly(ethylene-co-vinyl alcohol), containing 44 mol. % ethylene, had an imprinting effectiveness of more than six-fold. In random urine analysis, the microfluidic amperometric measurements of melatonin levels with an additional and recovery of melatonin, the melatonin recovery achieved 94.78± 1.9% for melatonin at a concentration of 1.75-2.11 pg/mL.

原文English
文章編號054115
期刊Biomicrofluidics
8
發行號5
DOIs
出版狀態Published - 2014 10月 15

All Science Journal Classification (ASJC) codes

  • 生物醫學工程
  • 材料科學(全部)
  • 凝聚態物理學
  • 流體流動和轉移過程
  • 膠體和表面化學

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