Monitoring bioactivity in hydrogen fermentation by an amperometric method with polyviologen modified working electrode

Ming Der Bai, Yung Fu Wang, Chia Jung Hsiao, Shiue Lin Li, Hsien Chang Chang, Sheng Shung Cheng

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

To monitor the bioactivity change of a hydrogen fermentor, this work presents a novel bioelectrochemical method equipped with a polyviologen modified glassy carbon as a working electrode and with ferricyanide as an electron mediator. Experimental results demonstrated that the ferricyanide can transfer electrons from hydrogen producing bacteria cells to electrode without significant inhibition on cell growth. To protect the electrode from fouling by protein and bacteria during bioassay, polyviologen film was applied to modify the working electrode, and stabilized the responding current compared to the use of bare electrode. The amperometric bioassay provided the bioactivity information for indicating the statue of the fermentor and helping draw up the operation strategy when the fermentor was operated under abnormal conditions, including stop-feeding and re-feeding. The results of bioactivity monitoring suggested an interesting phenomenon that the activity of hydrogen producing bacteria transiently increased during the unsteady period and then decreased.

原文English
頁(從 - 到)1357-1364
頁數8
期刊International Journal of Hydrogen Energy
31
發行號10
DOIs
出版狀態Published - 2006 八月

All Science Journal Classification (ASJC) codes

  • 可再生能源、永續發展與環境
  • 燃料技術
  • 凝聚態物理學
  • 能源工程與電力技術

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