Monitoring of geosmin producing Anabaena circinalis using quantitative PCR

Hsiang Wei Tsao, Atsuko Michinaka, Hung Kai Yen, Steven Giglio, Peter Hobson, Paul Monis, Tsair Fuh Lin

研究成果: Article同行評審

33 引文 斯高帕斯(Scopus)

摘要

Geosmin is one of the most commonly detected off-flavor chemicals present in reservoirs and drinking water systems. Quantitative real-time PCR (qPCR) is useful for quantifying geosmin-producers by focusing on the gene encoding geosmin synthase, which is responsible for geosmin synthesis. In this study, several primers and probes were designed and evaluated to detect the geosmin synthase gene in cyanobacteria. The specificity of primer and probe sets was tested using 21 strains of laboratory cultured cyanobacteria isolated from surface waters in Australia (18) and Taiwan (2), including 6 strains with geosmin producing ability. The results showed that the primers designed in this study could successfully detect all geosmin producing strains tested. The selected primers were used in a qPCR assay, and the calibration curves were linear from 5×101 to 5×105copiesmL-1, with a high correlation coefficient (R2=0.999). This method was then applied to analyze samples taken from Myponga Reservoir, South Australia, during a cyanobacterial bloom event. The results showed good correlations between qPCR techniques and traditional methods, including cell counts determined by microscopy and geosmin concentration measured using gas chromatography (GC) coupled with a mass selective detector (MSD). Results demonstrate that qPCR could be used for tracking geosmin-producing cyanobacteria in drinking water reservoirs. The qPCR assay may provide water utilities with the ability to properly characterize a taste and odor episode and choose appropriate management and treatment options.

原文English
頁(從 - 到)416-425
頁數10
期刊Water Research
49
DOIs
出版狀態Published - 2014 二月 1

All Science Journal Classification (ASJC) codes

  • 生態建模
  • 水科學與技術
  • 廢物管理和處置
  • 污染

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