Monitoring ribosomal frameshifting as a platform to screen anti-riboswitch drug candidates

Chien Hung Yu, René C.L. Olsthoorn

研究成果: Chapter

2 引文 斯高帕斯(Scopus)

摘要

Riboswitches are regions within mRNAs that can regulate downstream expression of genes through metabolite-induced alteration of their secondary structures. Due to the significant association of bacterial essential or virulence genes, bacterial riboswitches have become promising targets for development of putative antibacterial drugs. However, most of the screening systems to date are based on in vitro or bacterial systems, lacking the possibility to preobserve the adverse effects to the host's translation machinery. This chapter describes a novel screening method based on monitoring the riboswitch-induced - 1 ribosomal frameshifting (- 1 FS) efficiency in a mammalian cell-free lysate system using preQ1 class-I (preQ1-I) riboswitches as model target.

原文English
主出版物標題Methods in Enzymology
發行者Academic Press Inc.
頁面385-393
頁數9
DOIs
出版狀態Published - 2015

出版系列

名字Methods in Enzymology
550
ISSN(列印)0076-6879
ISSN(電子)1557-7988

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 分子生物學

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