Multiple vesiculoviral matrix proteins inhibit both nuclear export and import

Jeannine M. Petersen, Lu Shiun Her, James E. Dahlberg

研究成果: Article同行評審

51 引文 斯高帕斯(Scopus)


The matrix (M) protein of vesicular stomatitis virus inhibits both nuclear import and export. Here, we demonstrate that this inhibitory property is conserved between the M proteins from two other vesiculoviruses, chandipura virus and spring viremia carp virus. All three M proteins completely block nuclear transport of spliced mRNA, small nuclear RNAs, and small nuclear ribonucleoproteins and slow the nuclear transport of many other cargoes. In all cases where transport was merely slowed by the M proteins, the chandipura virus M protein had the strongest inhibitory activity. When expressed in transfected HeLa cells, active M proteins displayed prominent association with the nuclear rim. Moreover, mutation of a conserved methionine abolished both the inhibitory activity and efficient targeting of the M proteins to the nuclear rim. We propose that all of the vesiculoviral M proteins associate with the same nuclear target, which is likely to be a component of the nuclear pore complex.

頁(從 - 到)8590-8595
期刊Proceedings of the National Academy of Sciences of the United States of America
出版狀態Published - 2001 7月 17

All Science Journal Classification (ASJC) codes

  • 多學科


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