Muscarinic depression of excitatory synaptic transmission mediated by the presynaptic M3 receptors in the rat neostriatum

Kuei Sen Hsu, Chiung Chun Huang, Po Wu Gean

研究成果: Article同行評審

31 引文 斯高帕斯(Scopus)

摘要

The effect of carbachol on the excitatory synaptic transmission was studied in rat neostriatal neurons using intracellular and whole-cell voltage clamp-recording methods. Depolarizing excitatory postsynaptic potentials (EPSPs) were evoked by cortical stimulation. Superfusion of carbachol (0.01-3 μM) reversibly decreases the EPSP amplitude in a concentration-dependent manner and with an estimated IC50 of 0.3 μM. While, neither the N-methyl-d-aspartate (NMDA, 100 μM)- nor (±)-α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA, 100 μM)-induced response was affected by carbachol (0.1 μM). In addition, the inhibitory effect induced by carbachol at a low concentration of 0.1 μM. was attenuated by 4-diphenylacetoxy-N,N-methyl-piperidine (4-DAMP), a selective M3 muscarinic receptor antagonist. However, other muscarinic subtype (M1 or M2) antagonists could also block the inhibitory effect by carbachol 0.1 μM. The rank order of antagonist potency was: 4-DAMP (M3 antagonist) > methoctramine (M2 antagonist) > pirenzepine (M1 antagonist). Based on these findings, we conclude that carbachol at a low concentration (≤0.1 μM) reduced the excitatory response of neostriatal neurons following cortical stimulation via presynaptic M3 muscarinic receptors located on the terminals of corticostriatal neurons.

原文English
頁(從 - 到)141-144
頁數4
期刊Neuroscience Letters
197
發行號2
DOIs
出版狀態Published - 1995 九月 8

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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