Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold

Yu Cheng Lin, Hua Lin Wu

研究成果: Conference contribution

5 引文 斯高帕斯(Scopus)

摘要

We have broken the bottom limit of the melting temperature and denaturation temperature of amplicons of polymerase chain reactions (PCR) by adding Au nanoparticles. We found that the melting temperature and lowest denaturation temperature of PCR could be declined by adding Au nanoparticles. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the lowest melting temperatures and denaturation temperatures of the same DNA samples that could be amplified using a real-time PCR system were decreased by 1∼2°C and 1°C, respectively, compared with those without gold colloid added.

原文English
主出版物標題TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
頁面391-394
頁數4
DOIs
出版狀態Published - 2007
事件4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
持續時間: 2007 6月 102007 6月 14

出版系列

名字TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
國家/地區France
城市Lyon
期間07-06-1007-06-14

All Science Journal Classification (ASJC) codes

  • 控制與系統工程
  • 電氣與電子工程

指紋

深入研究「Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold」主題。共同形成了獨特的指紋。

引用此