Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold

研究成果: Conference contribution

2 引文 (Scopus)

摘要

We have broken the bottom limit of the melting temperature and denaturation temperature of amplicons of polymerase chain reactions (PCR) by adding Au nanoparticles. We found that the melting temperature and lowest denaturation temperature of PCR could be declined by adding Au nanoparticles. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the lowest melting temperatures and denaturation temperatures of the same DNA samples that could be amplified using a real-time PCR system were decreased by 1∼2°C and 1°C, respectively, compared with those without gold colloid added.

原文English
主出版物標題TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
頁面391-394
頁數4
DOIs
出版狀態Published - 2007 十二月 1
事件4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
持續時間: 2007 六月 102007 六月 14

出版系列

名字TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
國家France
城市Lyon
期間07-06-1007-06-14

指紋

Denaturation
Polymerase chain reaction
Melting point
Nanoparticles
Temperature
Colloids
DNA
Gold

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

引用此文

Lin, Y-C., & Wu, H-L. (2007). Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold. 於 TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (頁 391-394). [4300150] (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300150
Lin, Yu-Cheng ; Wu, Hua-Lin. / Nano-PCR : Breaking the bottom limit of the PCR denaturation temperature using nanogold. TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. 頁 391-394 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).
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abstract = "We have broken the bottom limit of the melting temperature and denaturation temperature of amplicons of polymerase chain reactions (PCR) by adding Au nanoparticles. We found that the melting temperature and lowest denaturation temperature of PCR could be declined by adding Au nanoparticles. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the lowest melting temperatures and denaturation temperatures of the same DNA samples that could be amplified using a real-time PCR system were decreased by 1∼2°C and 1°C, respectively, compared with those without gold colloid added.",
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Lin, Y-C & Wu, H-L 2007, Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold. 於 TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems., 4300150, TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, 頁 391-394, 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07, Lyon, France, 07-06-10. https://doi.org/10.1109/SENSOR.2007.4300150

Nano-PCR : Breaking the bottom limit of the PCR denaturation temperature using nanogold. / Lin, Yu-Cheng; Wu, Hua-Lin.

TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 391-394 4300150 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

研究成果: Conference contribution

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Lin Y-C, Wu H-L. Nano-PCR: Breaking the bottom limit of the PCR denaturation temperature using nanogold. 於 TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 391-394. 4300150. (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300150