Nanoparticle-assisted high efficient polymerase chain reaction

研究成果: Conference contribution

摘要

This paper reports that the Au nanoparticles could dramatically enhance efficiency of polymer chain reaction (PCR) and shorten reaction time. The excellent energy transfer property of the nanoparticles should be the major factor in improving the PCR efficiency. With the addition of 0.7 nM of 13 nm Au nanoparticles into the PCR reagent, the PCR efficiency was increased. The results demonstrated that Au nanoparticles increase the sensitivity of PCR detection 5-10 fold in a slower PCR system (i.e. conventional PCR) and at least 104 fold in a quicker PCR system (i.e. real-time PCR), This innovation could improve the PCR efficiency using non-expensive polymerases, and general PCR reagent.

原文English
主出版物標題2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008
頁面179-182
頁數4
DOIs
出版狀態Published - 2008 十二月 1
事件2008 ASME 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008 - Kowloon, Hong Kong
持續時間: 2008 六月 32008 六月 5

出版系列

名字2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008

Other

Other2008 ASME 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008
國家Hong Kong
城市Kowloon
期間08-06-0308-06-05

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Electrical and Electronic Engineering

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  • 引用此

    Lin, Y. C., & Wu, H. L. (2008). Nanoparticle-assisted high efficient polymerase chain reaction. 於 2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008 (頁 179-182). (2008 Proceedings of the ASME - 2nd International Conference on Integration and Commercialization of Micro and Nanosystems, MicroNano 2008). https://doi.org/10.1115/MicroNano2008-70214