Nanoparticle-mediated in-vitro gene transfection on the micro electroporation chip

Chun Ping Jen, Chun Sheng Fan, Yu-Cheng Lin, Yu-Hung Chen, Chia Wei Lee, Chen-Sheng Yeh

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

Gold nanoparticles modified with 21-based thiolated-oligonucleotides have been utilized as novel gene delivery vehicles for electroporation on microchips in the present study. The electroporation chip, consisting of a defined cell culture cavity region with thin film electrodes made of titanium and gold, was fabricated on a glass slide using microfabrication technologies. The electric field for the Au-DNA conjugates attracting was generated to induce the electrophoresis of Au-DNA conjugates and enhance the transfection efficient on the electroporation microchip. Both the UV-vis spectra of the surface plasmon resonance and TEM micrographs indicated that the nanoparticles associated with DNA could be delivered into cells utilizing electroporation. This investigation had demonstrated the nanoparticle-based gene delivery system.

原文English
主出版物標題TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
發行者Institute of Electrical and Electronics Engineers Inc.
頁面1047-1050
頁數4
ISBN(電子)0780377311, 9780780377318
DOIs
出版狀態Published - 2003 1月 1
事件12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States
持續時間: 2003 6月 82003 6月 12

出版系列

名字TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
2

Other

Other12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
國家/地區United States
城市Boston
期間03-06-0803-06-12

All Science Journal Classification (ASJC) codes

  • 電氣與電子工程

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