Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin: Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018

Yu Lin Lee, Min Chi Lu, Pei Lan Shao, Po Liang Lu, Yen Hsu Chen, Shu Hsing Cheng, Wen Chien Ko, Chi Ying Lin, Ting Shu Wu, Muh Yong Yen, Lih Shinn Wang, Chang Pan Liu, Wen Sen Lee, Zhi Yuan Shi, Yao Shen Chen, Fu Der Wang, Shu Hui Tseng, Chao Nan Lin, Yu Hui Chen, Wang Huei ShengChun Ming Lee, Ming Huei Liao, Po Ren Hsueh

研究成果: Article

摘要

Multicentre surveillance of antimicrobial susceptibility of clinically important Gram-negative bacteria (GNB) from 16 Taiwanese hospitals was performed. Escherichia coli (n = 398), Klebsiella pneumoniae (n = 346), Pseudomonas aeruginosa (n = 252) and Acinetobacter baumannii complex (ABC) (n = 188) bloodstream isolates, non-typhoidal Salmonella (n = 230) and Shigella flexneri (n = 18) from various sources were collected. Antimicrobial MICs were determined using broth microdilution. Genes encoding K. pneumoniae carbapenemases (KPCs), New Delhi metallo-β-lactamases (NDMs), Verona integron-encoded metallo-β-lactamase (VIM), OXA-48-like carbapenemase (OXA-48) as well as mcr-1–5 genes were detected by molecular methods. Rates of carbapenem non-susceptibility were 2.8%, 9.0%, 0.4%, 0%, 10.3% and 48.8% for E. coli, K. pneumoniae, Salmonella, Shigella, P. aeruginosa and ABC, respectively. For carbapenemases, one (0.3%) E. coli harboured blaNDM-1. Fifteen (4.3%), two (0.6%) and two (0.6%) K. pneumoniae contained blaKPC, blaOXA-48 and blaVIM, respectively. Two (0.5%) E. coli and fourteen (4.0%) K. pneumoniae were non-wild-type according to the colistin MIC. Among Enterobacteriaceae with a colistin MIC ≥ 2 mg/L, mcr-1 was detected in one E. coli, two K. pneumoniae and three Salmonella spp. All three mcr-1-positive Salmonella isolates were collected from community-acquired infections; none of the six mcr-1-positive Enterobacteriaceae were carbapenem-resistant. Carbapenem resistance has increased among clinically important GNB, especially among hospital-acquired infections. blaKPC, especially the blaKPC-2 variant, was detected in approximately one-half of the carbapenem-resistant K. pneumoniae isolates in this study. Although resistance rates to colistin remained low among Enterobacteriaceae, the finding of mcr-1 from different species raises concern of potential dissemination.

原文English
頁(從 - 到)318-328
頁數11
期刊International journal of antimicrobial agents
54
發行號3
DOIs
出版狀態Published - 2019 九月

指紋

Colistin
Carbapenems
Klebsiella pneumoniae
Gram-Negative Bacteria
Taiwan
Salmonella
Escherichia coli
Enterobacteriaceae
Acinetobacter baumannii
Pseudomonas aeruginosa
Integrons
Community-Acquired Infections
Shigella flexneri
Shigella
Cross Infection
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

引用此文

Lee, Yu Lin ; Lu, Min Chi ; Shao, Pei Lan ; Lu, Po Liang ; Chen, Yen Hsu ; Cheng, Shu Hsing ; Ko, Wen Chien ; Lin, Chi Ying ; Wu, Ting Shu ; Yen, Muh Yong ; Wang, Lih Shinn ; Liu, Chang Pan ; Lee, Wen Sen ; Shi, Zhi Yuan ; Chen, Yao Shen ; Wang, Fu Der ; Tseng, Shu Hui ; Lin, Chao Nan ; Chen, Yu Hui ; Sheng, Wang Huei ; Lee, Chun Ming ; Liao, Ming Huei ; Hsueh, Po Ren. / Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin : Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018. 於: International journal of antimicrobial agents. 2019 ; 卷 54, 編號 3. 頁 318-328.
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title = "Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin: Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018",
abstract = "Multicentre surveillance of antimicrobial susceptibility of clinically important Gram-negative bacteria (GNB) from 16 Taiwanese hospitals was performed. Escherichia coli (n = 398), Klebsiella pneumoniae (n = 346), Pseudomonas aeruginosa (n = 252) and Acinetobacter baumannii complex (ABC) (n = 188) bloodstream isolates, non-typhoidal Salmonella (n = 230) and Shigella flexneri (n = 18) from various sources were collected. Antimicrobial MICs were determined using broth microdilution. Genes encoding K. pneumoniae carbapenemases (KPCs), New Delhi metallo-β-lactamases (NDMs), Verona integron-encoded metallo-β-lactamase (VIM), OXA-48-like carbapenemase (OXA-48) as well as mcr-1–5 genes were detected by molecular methods. Rates of carbapenem non-susceptibility were 2.8{\%}, 9.0{\%}, 0.4{\%}, 0{\%}, 10.3{\%} and 48.8{\%} for E. coli, K. pneumoniae, Salmonella, Shigella, P. aeruginosa and ABC, respectively. For carbapenemases, one (0.3{\%}) E. coli harboured blaNDM-1. Fifteen (4.3{\%}), two (0.6{\%}) and two (0.6{\%}) K. pneumoniae contained blaKPC, blaOXA-48 and blaVIM, respectively. Two (0.5{\%}) E. coli and fourteen (4.0{\%}) K. pneumoniae were non-wild-type according to the colistin MIC. Among Enterobacteriaceae with a colistin MIC ≥ 2 mg/L, mcr-1 was detected in one E. coli, two K. pneumoniae and three Salmonella spp. All three mcr-1-positive Salmonella isolates were collected from community-acquired infections; none of the six mcr-1-positive Enterobacteriaceae were carbapenem-resistant. Carbapenem resistance has increased among clinically important GNB, especially among hospital-acquired infections. blaKPC, especially the blaKPC-2 variant, was detected in approximately one-half of the carbapenem-resistant K. pneumoniae isolates in this study. Although resistance rates to colistin remained low among Enterobacteriaceae, the finding of mcr-1 from different species raises concern of potential dissemination.",
author = "Lee, {Yu Lin} and Lu, {Min Chi} and Shao, {Pei Lan} and Lu, {Po Liang} and Chen, {Yen Hsu} and Cheng, {Shu Hsing} and Ko, {Wen Chien} and Lin, {Chi Ying} and Wu, {Ting Shu} and Yen, {Muh Yong} and Wang, {Lih Shinn} and Liu, {Chang Pan} and Lee, {Wen Sen} and Shi, {Zhi Yuan} and Chen, {Yao Shen} and Wang, {Fu Der} and Tseng, {Shu Hui} and Lin, {Chao Nan} and Chen, {Yu Hui} and Sheng, {Wang Huei} and Lee, {Chun Ming} and Liao, {Ming Huei} and Hsueh, {Po Ren}",
year = "2019",
month = "9",
doi = "10.1016/j.ijantimicag.2019.06.009",
language = "English",
volume = "54",
pages = "318--328",
journal = "International Journal of Antimicrobial Agents",
issn = "0924-8579",
publisher = "Elsevier",
number = "3",

}

Lee, YL, Lu, MC, Shao, PL, Lu, PL, Chen, YH, Cheng, SH, Ko, WC, Lin, CY, Wu, TS, Yen, MY, Wang, LS, Liu, CP, Lee, WS, Shi, ZY, Chen, YS, Wang, FD, Tseng, SH, Lin, CN, Chen, YH, Sheng, WH, Lee, CM, Liao, MH & Hsueh, PR 2019, 'Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin: Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018', International journal of antimicrobial agents, 卷 54, 編號 3, 頁 318-328. https://doi.org/10.1016/j.ijantimicag.2019.06.009

Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin : Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018. / Lee, Yu Lin; Lu, Min Chi; Shao, Pei Lan; Lu, Po Liang; Chen, Yen Hsu; Cheng, Shu Hsing; Ko, Wen Chien; Lin, Chi Ying; Wu, Ting Shu; Yen, Muh Yong; Wang, Lih Shinn; Liu, Chang Pan; Lee, Wen Sen; Shi, Zhi Yuan; Chen, Yao Shen; Wang, Fu Der; Tseng, Shu Hui; Lin, Chao Nan; Chen, Yu Hui; Sheng, Wang Huei; Lee, Chun Ming; Liao, Ming Huei; Hsueh, Po Ren.

於: International journal of antimicrobial agents, 卷 54, 編號 3, 09.2019, p. 318-328.

研究成果: Article

TY - JOUR

T1 - Nationwide surveillance of antimicrobial resistance among clinically important Gram-negative bacteria, with an emphasis on carbapenems and colistin

T2 - Results from the Surveillance of Multicenter Antimicrobial Resistance in Taiwan (SMART) in 2018

AU - Lee, Yu Lin

AU - Lu, Min Chi

AU - Shao, Pei Lan

AU - Lu, Po Liang

AU - Chen, Yen Hsu

AU - Cheng, Shu Hsing

AU - Ko, Wen Chien

AU - Lin, Chi Ying

AU - Wu, Ting Shu

AU - Yen, Muh Yong

AU - Wang, Lih Shinn

AU - Liu, Chang Pan

AU - Lee, Wen Sen

AU - Shi, Zhi Yuan

AU - Chen, Yao Shen

AU - Wang, Fu Der

AU - Tseng, Shu Hui

AU - Lin, Chao Nan

AU - Chen, Yu Hui

AU - Sheng, Wang Huei

AU - Lee, Chun Ming

AU - Liao, Ming Huei

AU - Hsueh, Po Ren

PY - 2019/9

Y1 - 2019/9

N2 - Multicentre surveillance of antimicrobial susceptibility of clinically important Gram-negative bacteria (GNB) from 16 Taiwanese hospitals was performed. Escherichia coli (n = 398), Klebsiella pneumoniae (n = 346), Pseudomonas aeruginosa (n = 252) and Acinetobacter baumannii complex (ABC) (n = 188) bloodstream isolates, non-typhoidal Salmonella (n = 230) and Shigella flexneri (n = 18) from various sources were collected. Antimicrobial MICs were determined using broth microdilution. Genes encoding K. pneumoniae carbapenemases (KPCs), New Delhi metallo-β-lactamases (NDMs), Verona integron-encoded metallo-β-lactamase (VIM), OXA-48-like carbapenemase (OXA-48) as well as mcr-1–5 genes were detected by molecular methods. Rates of carbapenem non-susceptibility were 2.8%, 9.0%, 0.4%, 0%, 10.3% and 48.8% for E. coli, K. pneumoniae, Salmonella, Shigella, P. aeruginosa and ABC, respectively. For carbapenemases, one (0.3%) E. coli harboured blaNDM-1. Fifteen (4.3%), two (0.6%) and two (0.6%) K. pneumoniae contained blaKPC, blaOXA-48 and blaVIM, respectively. Two (0.5%) E. coli and fourteen (4.0%) K. pneumoniae were non-wild-type according to the colistin MIC. Among Enterobacteriaceae with a colistin MIC ≥ 2 mg/L, mcr-1 was detected in one E. coli, two K. pneumoniae and three Salmonella spp. All three mcr-1-positive Salmonella isolates were collected from community-acquired infections; none of the six mcr-1-positive Enterobacteriaceae were carbapenem-resistant. Carbapenem resistance has increased among clinically important GNB, especially among hospital-acquired infections. blaKPC, especially the blaKPC-2 variant, was detected in approximately one-half of the carbapenem-resistant K. pneumoniae isolates in this study. Although resistance rates to colistin remained low among Enterobacteriaceae, the finding of mcr-1 from different species raises concern of potential dissemination.

AB - Multicentre surveillance of antimicrobial susceptibility of clinically important Gram-negative bacteria (GNB) from 16 Taiwanese hospitals was performed. Escherichia coli (n = 398), Klebsiella pneumoniae (n = 346), Pseudomonas aeruginosa (n = 252) and Acinetobacter baumannii complex (ABC) (n = 188) bloodstream isolates, non-typhoidal Salmonella (n = 230) and Shigella flexneri (n = 18) from various sources were collected. Antimicrobial MICs were determined using broth microdilution. Genes encoding K. pneumoniae carbapenemases (KPCs), New Delhi metallo-β-lactamases (NDMs), Verona integron-encoded metallo-β-lactamase (VIM), OXA-48-like carbapenemase (OXA-48) as well as mcr-1–5 genes were detected by molecular methods. Rates of carbapenem non-susceptibility were 2.8%, 9.0%, 0.4%, 0%, 10.3% and 48.8% for E. coli, K. pneumoniae, Salmonella, Shigella, P. aeruginosa and ABC, respectively. For carbapenemases, one (0.3%) E. coli harboured blaNDM-1. Fifteen (4.3%), two (0.6%) and two (0.6%) K. pneumoniae contained blaKPC, blaOXA-48 and blaVIM, respectively. Two (0.5%) E. coli and fourteen (4.0%) K. pneumoniae were non-wild-type according to the colistin MIC. Among Enterobacteriaceae with a colistin MIC ≥ 2 mg/L, mcr-1 was detected in one E. coli, two K. pneumoniae and three Salmonella spp. All three mcr-1-positive Salmonella isolates were collected from community-acquired infections; none of the six mcr-1-positive Enterobacteriaceae were carbapenem-resistant. Carbapenem resistance has increased among clinically important GNB, especially among hospital-acquired infections. blaKPC, especially the blaKPC-2 variant, was detected in approximately one-half of the carbapenem-resistant K. pneumoniae isolates in this study. Although resistance rates to colistin remained low among Enterobacteriaceae, the finding of mcr-1 from different species raises concern of potential dissemination.

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EP - 328

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

SN - 0924-8579

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