Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla setrata

Li Li Chen, Chu Fang Lo, Ya Lin Chiu, Chen Fang Chang, Guang Hsiung Kou

研究成果: Article同行評審

132 引文 斯高帕斯(Scopus)

摘要

White spot syndrome virus (WSSV), the causative agent of white spot syndrome in shrimp, has a wide host range which extends to crabs, copepods and other arthropods. In this study, benthic larvae of the mud crab Scylla serrata were captured from Taiwan's coastal waters and screened for the presence of WSSV by polymerase chain reaction (PCR) and in situ hybridization. WSSV was detected in around 60% of the larvae, and this prevalence rate remained fairly constant when the captured larvae were subsequently maintained in an aerated system in the laboratory. WSSV-free larvae obtained from a hatchery were challenged by immersion in a WSSV inoculum. Fifteen days after challenge, cumulative mortality in the experimental group reached 43% compared to 20% in the control group. PCR detection of WSSV in both moribund and surviving specimens clearly implicated the virus as the cause of death in most cases. Histological and in situ hybridization data confirmed that WSSV tissue tropism in Scylla setrata crab larvae is similar to that found in shrimp.

原文English
頁(從 - 到)157-161
頁數5
期刊Diseases of Aquatic Organisms
40
發行號2
DOIs
出版狀態Published - 2000 三月 14

All Science Journal Classification (ASJC) codes

  • 生態學、進化論、行為學與系統學
  • 海洋科學

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