Neuronal morphological change of size-sieved stem cells induced by neurotrophic stimuli

Shun Fen Tzeng, May J. Tsai, Shih Chieh Hung, Henrich Cheng

研究成果: Article同行評審

20 引文 斯高帕斯(Scopus)

摘要

Size-sieved stem cells (SSCs) derived from human bone marrow have the ability to differentiate into bone, fat and cartilage. SSCs can differentiate into active neural cells after exposure to antioxidant agents. The aim of the present study is to understand if SSCs can be stimulated to differentiate into neurons in response to neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), pituitary adenylate cyclase-activating polypeptide (PACAP) and dibutyryl cAMP (dbcAMP). SSCs in a serum-free medium transform from a fibroblastic-like form to a multipolar morphology. Treatment of SSCs with GDNF, PACAP, and dbcAMP increased the production of neurofilament light protein (NF-L) and a cytoskeleton protein-α-tubulin. Examination of a vesicle protein-synapsin-1 or a neuronal progenitor marker-internexin in SSCs indicated that treatment with GDNF, PACAP, and dbcAMP further elongated cell processes and increased process branching. The findings indicate that neurotrophic signaling and cAMP-dependent signaling might promote the neuronal differentiation of SSCs.

原文English
頁(從 - 到)23-28
頁數6
期刊Neuroscience Letters
367
發行號1
DOIs
出版狀態Published - 2004 八月 26

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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