New isoflavonoid glycosides and related constituents from astragali radix (astragalus membranaceus) and their inhibitory activity on nitric oxide production

Li Jie Zhang, Hui Kang Liu, Ping Chun Hsiao, Li Ming Yang Kuo, I. Jung Lee, Tian Shung Wu, Wen Fei Chiou, Yao Haur Kuo

研究成果: Article同行評審

77 引文 斯高帕斯(Scopus)

摘要

Twenty-four secondary metabolites, including 16 isoflavonoids, 7 astragalasides, and 1 benzoquinone, have been isolated from the roots of Astragalus membranaceus (Astragali radix). Among these isolated isoflavonoids, (-)-methylinissolin 3-O-β-d-(6′-acetyl)-glucoside (1), (-)-methylinissolin 3-O-β-d-{6′-[(E)-but-2-enoyl]}-glucoside (2), and calycosin 7-O-β-d-(6″-acetyl)-glucoside (3) have been identified as new compounds on the basis of spectroscopic analysis; (-)-methylinissolin 3-O-β-d-glucoside (4) was isolated from the natural products for the first time. The nitric oxide (NO) production inhibitory activity of the major compounds has been assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. To identify A. membranaceus, a fingerprint method was developed by using a high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method. Furthermore, characteristic peaks for the 11 major compounds in the chromatogram were unambiguously confirmed.

原文English
頁(從 - 到)1131-1137
頁數7
期刊Journal of Agricultural and Food Chemistry
59
發行號4
DOIs
出版狀態Published - 2011 2月 23

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 農業與生物科學 (全部)

指紋

深入研究「New isoflavonoid glycosides and related constituents from astragali radix (astragalus membranaceus) and their inhibitory activity on nitric oxide production」主題。共同形成了獨特的指紋。

引用此