Noncoding effects of circular RNA CCDC66 promote colon cancer growth and metastasis

Kuei Yang Hsiao, Ya Chi Lin, Sachin Kumar Gupta, Ning Chang, Laising Yen, H. Sunny Sun, Shaw Jenq Tsai

研究成果: Article

155 引文 (Scopus)

摘要

Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly unknown. Recent studies indicate circ-RNA may be involved in disease pathogenesis, but direct evidence is scarce. Here, we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-offunction studies in colorectal cancer cell lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis.

原文English
頁(從 - 到)2339-2350
頁數12
期刊Cancer Research
77
發行號9
DOIs
出版狀態Published - 2017 五月 1

指紋

Colonic Neoplasms
Neoplasm Metastasis
Growth
Neoplasms
Colorectal Neoplasms
RNA
Untranslated RNA
Pathologic Processes
Polyps
Oncogenes
Heterografts
Cell Movement
Colon
Cell Proliferation
circular RNA
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

引用此文

Hsiao, Kuei Yang ; Lin, Ya Chi ; Gupta, Sachin Kumar ; Chang, Ning ; Yen, Laising ; Sun, H. Sunny ; Tsai, Shaw Jenq. / Noncoding effects of circular RNA CCDC66 promote colon cancer growth and metastasis. 於: Cancer Research. 2017 ; 卷 77, 編號 9. 頁 2339-2350.
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Noncoding effects of circular RNA CCDC66 promote colon cancer growth and metastasis. / Hsiao, Kuei Yang; Lin, Ya Chi; Gupta, Sachin Kumar; Chang, Ning; Yen, Laising; Sun, H. Sunny; Tsai, Shaw Jenq.

於: Cancer Research, 卷 77, 編號 9, 01.05.2017, p. 2339-2350.

研究成果: Article

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AU - Sun, H. Sunny

AU - Tsai, Shaw Jenq

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AB - Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly unknown. Recent studies indicate circ-RNA may be involved in disease pathogenesis, but direct evidence is scarce. Here, we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-offunction studies in colorectal cancer cell lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis.

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