Noninvasive monitoring of embryonic stem cells in vivo with MRI transgene reporter

Jun Liu, Eric C.H. Cheng, Robert C. Long, Shang Hsun Yang, Liya Wang, Pei Hsun Cheng, Jinjing Yang, Dong Wu, Hui Mao, Anthony W.S. Chan

研究成果: Article同行評審

63 引文 斯高帕斯(Scopus)

摘要

Reporter gene-based magnetic resonance imaging (MRI) offers unique insights into behavior of cells after transplantation, which could significantly benefit stem cell research and translation. Several candidate MRI reporter genes, including one that encodes for iron storage protein ferritin, have been reported, and their potential applications in embryonic stem (ES) cell research have yet to be explored. We have established transgenic mouse ES (mES) cell lines carrying human ferritin heavy chain (FTH) as a reporter gene and succeeded in monitoring the cell grafts in vivo using T2-weighted MRI sequences. FTH generated MRI contrast through compensatory upregulation of transferrin receptor (Tfrc) that led to increased cellular iron stored in ferritin-bound form. At a level sufficient for MRI contrast, expression of FTH posed no toxicity to mES cells and did not interfere with stem cell pluripotency as observed in neural differentiation and teratoma formation. The compatibility and functionality of ferritin as a reporter in mES cells opens up the possibility of using MRI for longitudinal noninvasive monitoring of ES cell-derived cell grafts at both molecular and cellular levels.

原文English
頁(從 - 到)739-747
頁數9
期刊Tissue Engineering - Part C: Methods
15
發行號4
DOIs
出版狀態Published - 2009 12月 1

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 醫藥(雜項)
  • 生物醫學工程

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