Novel flow cytometrer utilizing wavelength-resovled detection under a diascopic illumination configuration

Shi Wei Lin, Chih-Han Chang, Chia Yen Lee, Lung-Ming Fu, Che Hsin Lin

研究成果: Conference contribution

2 引文 斯高帕斯(Scopus)

摘要

This paper presents a novel yet high performance diascopic illumination configuration for simultaneously detecting cells of different sizes and different fluorescence labeling in a microfluidic chip. An objective-type dark-field condenser equipped with a low-cost tungsten bulb light source with continuous wavelengths (400 to 900 nm) and an UV-Vis-NIR spectrometer is used for detecting the multispectral signals from various particles and cells. With this approach, continuous cell counting and spectra analyzing are realized in a single channel without using any spatial filter and delicate optical detectors. Results show that the developed system is capable of identifying different labeled particle and cell samples by extracting the information side-scatter, absorption, and fluorescence from the information-rich spectra. This proposed system provides an efficient multi-color detection for identification and classification on a micro-flow cytometer chip.

原文English
主出版物標題IEEE Sensors 2010 Conference, SENSORS 2010
頁面1987-1990
頁數4
DOIs
出版狀態Published - 2010 12月 1
事件9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
持續時間: 2010 11月 12010 11月 4

出版系列

名字Proceedings of IEEE Sensors

Other

Other9th IEEE Sensors Conference 2010, SENSORS 2010
國家/地區United States
城市Waikoloa, HI
期間10-11-0110-11-04

All Science Journal Classification (ASJC) codes

  • 電氣與電子工程

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