Novel subunit vaccine with linear array epitope protect giant grouper against nervous necrosis virus infection

Chao Fen Lin, Han Kai Jiang, Nai Chi Chen, Ting Yu Wang, Tzong Yueh Chen

研究成果: Article

3 引文 (Scopus)

摘要

Viral nervous necrosis caused by nervous necrosis virus (NNV) is one of the most severe diseases resulting in high fish mortality rates and high economic losses in the giant grouper industry. Various NNV vaccines have been evaluated, such as inactivated viruses, virus-like particles (VLPs), recombinant coat proteins, synthetic peptides of coat proteins, and DNA vaccines. However, a cheaper manufacturing process and effective protection of NNV vaccines for commercial application are yet to be established. Hence, the present study developed a novel subunit vaccine composed of a carrier protein, receptor-binding domain of Pseudomonas exotoxin A, and tandem-repeated NNV coat protein epitopes by using the structural basis of epitope prediction and the linear array epitope (LAE) technique. On the basis of the crystal structure of the NNV coat protein, the epitope was predicted from the putative target cell receptor-binding region to elicit neutralizing immune responses. The safety of the LAE vaccine was evaluated, and all vaccinated fish survived without any physiological changes. The coat protein-specific antibody titers in the vaccinated fish increased after vaccine administration and exerted NNV-neutralizing effects. The efficacy tests revealed that the relative percent survival (RPS) of LAE antigen formulated with adjuvant was above 72% and LAE vaccine was effective for preventing NNV infection in giant grouper. This study is the first to develop an NNV vaccine by using epitope repeats, which provided effective protection to giant grouper against virus infection. The LAE construct can be used as a vaccine design platform against various pathogenic diseases.

原文English
頁(從 - 到)551-558
頁數8
期刊Fish and Shellfish Immunology
74
DOIs
出版狀態Published - 2018 三月 1

指紋

Subunit Vaccines
grouper
subunit vaccines
vaccine
Viruses
epitopes
Epitopes
virus
necrosis
viruses
coat proteins
Vaccines
Capsid Proteins
vaccines
infection
protein
Fish
neutralization
fish
exotoxins

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

引用此文

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abstract = "Viral nervous necrosis caused by nervous necrosis virus (NNV) is one of the most severe diseases resulting in high fish mortality rates and high economic losses in the giant grouper industry. Various NNV vaccines have been evaluated, such as inactivated viruses, virus-like particles (VLPs), recombinant coat proteins, synthetic peptides of coat proteins, and DNA vaccines. However, a cheaper manufacturing process and effective protection of NNV vaccines for commercial application are yet to be established. Hence, the present study developed a novel subunit vaccine composed of a carrier protein, receptor-binding domain of Pseudomonas exotoxin A, and tandem-repeated NNV coat protein epitopes by using the structural basis of epitope prediction and the linear array epitope (LAE) technique. On the basis of the crystal structure of the NNV coat protein, the epitope was predicted from the putative target cell receptor-binding region to elicit neutralizing immune responses. The safety of the LAE vaccine was evaluated, and all vaccinated fish survived without any physiological changes. The coat protein-specific antibody titers in the vaccinated fish increased after vaccine administration and exerted NNV-neutralizing effects. The efficacy tests revealed that the relative percent survival (RPS) of LAE antigen formulated with adjuvant was above 72{\%} and LAE vaccine was effective for preventing NNV infection in giant grouper. This study is the first to develop an NNV vaccine by using epitope repeats, which provided effective protection to giant grouper against virus infection. The LAE construct can be used as a vaccine design platform against various pathogenic diseases.",
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Novel subunit vaccine with linear array epitope protect giant grouper against nervous necrosis virus infection. / Lin, Chao Fen; Jiang, Han Kai; Chen, Nai Chi; Wang, Ting Yu; Chen, Tzong Yueh.

於: Fish and Shellfish Immunology, 卷 74, 01.03.2018, p. 551-558.

研究成果: Article

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AU - Chen, Tzong Yueh

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