On-chip, aptamer-based sandwich assay for detection of glycated hemoglobins via magnetic beads

Jinglun Li, Ko Wei Chang, Chih Hung Wang, Ching Hsuan Yang, Shu Chu Shiesh, Gwo Bin Lee

研究成果: Article同行評審

40 引文 斯高帕斯(Scopus)

摘要

Diabetes can be diagnosed and monitored by measurement of the cutoff ratio between glycated hemoglobins (HbA1c) and total hemoglobin (Hb), which does not require a fasting blood sample and is less influenced by biological variations. In this study, we combined the advantages of the microfluidic system and the selected low-cost, stable and specific aptamers and developed an integrated, aptamer-based microfluidic system for automatic glycated hemoglobin measurements. The detection process of human whole blood can be totally automated in this integrated microfluidic system. According to the experimental results, when compared to conventional bench-top manual assays, reagent consumption was significantly reduced by 75%, and the analysis time was reduced from 3.5 h to 30 min. Besides, the novelty in this research also lies in the simultaneously performed two parallel assays for detection of Hb and HbA1c in a single chip. Therefore, this sensitive and low-cost aptamer-based microfluidic system may become a promising tool for point-of -care diagnosis of diabetes.

原文English
頁(從 - 到)887-893
頁數7
期刊Biosensors and Bioelectronics
79
DOIs
出版狀態Published - 2016 五月 15

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物物理學
  • 生物醫學工程
  • 電化學

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