On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

Yao Nan Wang; Jik Chang Leong; Chin Lung Chang; Chang Hsien Tai; Chien Hsiung Tsai; Lung Ming Fu; Jr Ming Miao

doi:10.1115/MNHMT2009-18298

On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

Yao Nan Wang
, Jik Chang Leong
, Chin Lung Chang
, Chang Hsien Tai
, Chien Hsiung Tsai
, Lung Ming Fu
, Jr Ming Miao

工程科學系

研究成果: Conference contribution

摘要

In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.

原文	English
主出版物標題	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
頁面	137-142
頁數	6
DOIs	https://doi.org/10.1115/MNHMT2009-18298
出版狀態	Published - 2010 7月 12
事件	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 - Shanghai, China 持續時間: 2009 12月 18 → 2009 12月 21

出版系列

名字	Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
卷	1

Other

Other	ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
國家/地區	China
城市	Shanghai
期間	09-12-18 → 09-12-21

All Science Journal Classification (ASJC) codes

流體流動和轉移過程

存取文件

10.1115/MNHMT2009-18298

其它文件與鏈接

引用此

Wang, Y. N., Leong, J. C., Chang, C. L., Tai, C. H., Tsai, C. H., Fu, L. M., & Miao, J. M. (2010). On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. 於 Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 (頁 137-142). (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; 卷 1). https://doi.org/10.1115/MNHMT2009-18298

Wang, Yao Nan ; Leong, Jik Chang ; Chang, Chin Lung 等. / On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. 頁 137-142 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009).

@inproceedings{b5cd392f595741ac8a45e52b7afb0105,

title = "On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing",

abstract = "In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.",

author = "Wang, \{Yao Nan\} and Leong, \{Jik Chang\} and Chang, \{Chin Lung\} and Tai, \{Chang Hsien\} and Tsai, \{Chien Hsiung\} and Fu, \{Lung Ming\} and Miao, \{Jr Ming\}",

year = "2010",

month = jul,

day = "12",

doi = "10.1115/MNHMT2009-18298",

language = "English",

isbn = "9780791843895",

series = "Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009",

pages = "137--142",

booktitle = "Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009",

note = "ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 ; Conference date: 18-12-2009 Through 21-12-2009",

}

Wang, YN, Leong, JC, Chang, CL, Tai, CH, Tsai, CH, Fu, LM & Miao, JM 2010, On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. 於 Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, 卷 1, 頁 137-142, ASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009, Shanghai, China, 09-12-18. https://doi.org/10.1115/MNHMT2009-18298

On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. / Wang, Yao Nan; Leong, Jik Chang; Chang, Chin Lung 等.
Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 137-142 (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009; 卷 1).

研究成果: Conference contribution

TY - GEN

T1 - On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

AU - Wang, Yao Nan

AU - Leong, Jik Chang

AU - Chang, Chin Lung

AU - Tai, Chang Hsien

AU - Tsai, Chien Hsiung

AU - Fu, Lung Ming

AU - Miao, Jr Ming

PY - 2010/7/12

Y1 - 2010/7/12

N2 - In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.

AB - In this paper, a microflow cytometer for particles/cells passive focusing, fluorescence detection, and sizing is demonstrated with a convergent geometry of 50 μm detection gate channel and a pair of external optic fibers on poly-dimethylsiloxane (PDMS) microchip. A laser (635 nm) is used for fluorescent excitation. The amplitudes of the backward fluorescence signal and forward scattered light signal indicate the corresponding fluorescent intensity and size of the detected fluorescent particles and labeled white blood cells, respectively. In the experiment, the uniform fluorescent light intensity was measured by focusing the fluorescent particles in a 200×50×30μm detection gate channel. However, the emitted fluorescence and forward scattered light intensity of different size particles/cells are also measured simultaneously by external optic fibers.

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M3 - Conference contribution

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Wang YN, Leong JC, Chang CL, Tai CH, Tsai CH, Fu LM 等. On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing. 於 Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009. 2010. p. 137-142. (Proceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009). doi: 10.1115/MNHMT2009-18298

On-chip particle differentiation utilizing forward scattered light and fluorescence light with passive focusing

摘要

出版系列

Other

All Science Journal Classification (ASJC) codes

存取文件

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