One-step transformation of yeast in stationary phase

Dz Chi Chen, Bei-Chang Yang, Tsong Teh Kuo

研究成果: Article

523 引文 (Scopus)

摘要

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

原文English
頁(從 - 到)83-84
頁數2
期刊Current Genetics
21
發行號1
DOIs
出版狀態Published - 1992 一月 1

指紋

Yeasts
DNA
Alkalies
Plasmids
Ions

All Science Journal Classification (ASJC) codes

  • Genetics

引用此文

Chen, Dz Chi ; Yang, Bei-Chang ; Kuo, Tsong Teh. / One-step transformation of yeast in stationary phase. 於: Current Genetics. 1992 ; 卷 21, 編號 1. 頁 83-84.
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One-step transformation of yeast in stationary phase. / Chen, Dz Chi; Yang, Bei-Chang; Kuo, Tsong Teh.

於: Current Genetics, 卷 21, 編號 1, 01.01.1992, p. 83-84.

研究成果: Article

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