Optimisation of biomass and lipid production of a tropical thraustochytrid Aurantiochytrium sp. UMACC-T023 in submerged-liquid fermentation for large-scale biodiesel production

Mohamad Nor Azzimi Sohedein, Wan Abd Al Qadr Imad Wan-Mohtar, Yeong Hui-Yin, Zul Ilham, Jo Shu Chang, Sugenendran Supramani, Phang Siew-Moi

研究成果: Article同行評審

12 引文 斯高帕斯(Scopus)

摘要

A tropical thraustochytrid, Aurantiochytrium sp. UMACC-T023, was optimised for high biomass and lipid production in submerged-liquid fermentation (SLF). Biomass and lipid production were optimised based on glucose, polypeptone, and yeast extract concentration using response surface methodology (RSM). In RSM, the applied central composite design (CCD) showed that the optimisation model was significant for all variables studied. Yeast extract and polypeptone were associated with significant effects in UMACC-T023 biomass production with p < 0.05. The model was validated by employing the optimised media composition in shake flasks and in 1.4-L stirred-tank bioreactors. The optimised media composition for both biomass and lipid production was 10 g L−1 glucose, 15.08 g L−1 polypeptone, and 13.56 g L−1 yeast extract. Biomass production in the bioreactor increased 2.12-fold compared with the shake flask culture utilising the same optimised media composition. This study demonstrates the potential of large-scale lipid production of UMACC-T023 as biodiesel feedstock.

原文English
文章編號101496
期刊Biocatalysis and Agricultural Biotechnology
23
DOIs
出版狀態Published - 2020 1月

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物工程
  • 食品科學
  • 應用微生物與生物技術
  • 農學與作物科學

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