Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement

Wei Teh Jiang, Yolin Tsai, Xisen Wang, Zhaohui Li

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

As a cationic fluorescence dye acridine orange (AO) is commonly used in biology and biochemistry for DNA analyses. In this study the light absorption and fluorescence of AO after being sorbed on kaolinite (Kao) and halloysite (Hal) were investigated. In dilute systems with initial AO concentrations of 1 × 10−6 to 1 × 10−4 M (0.3–25 μmol/g loadings on Kao), both light absorption and fluorescence emission increased as the initial AO concentrations, thus, the amounts of AO sorbed increased. In contrast, the light absorption and fluorescence emission reached maxima at 1 × 10−5 M (3 μmol/g) for Hal. In concentrated systems with initial AO concentrations of 5 × 10−4–5 × 10−3 M (22–57 and 50–126 μmol/g AO sorption on Kao and Hal), significant fluorescence quenching was observed and the fluorescence intensity decreased as the initial AO concentrations, thus, the amounts of AO sorbed increased. The results suggested that to achieve maximal fluorescence emission, monomeric AO configuration on the solid surface is a necessity. The AO sorption was mostly attributed to cation exchange between protonated AO and exchangeable cations on Kao and Hal surfaces. As such, both cation exchange capacity and specific surface area of the minerals control the maximal fluorescence emission.

原文English
頁(從 - 到)407-418
頁數12
期刊Journal of Industrial and Engineering Chemistry
90
DOIs
出版狀態Published - 2020 10月 25

All Science Journal Classification (ASJC) codes

  • 化學工程 (全部)

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