Optimization of an electroporation system for gene transfection by taguchi method

Yu Cheng Lin, Ching Yuan Hung, Keng Shiang Huang, Chun Sheng Fang, Chih Hui Yang

研究成果: Conference contribution

摘要

This study demonstrates an optimized electroporation (EP) system for gene transfection by the Taguchi method. The parameters of the EP system, having an influence on the transfection rate, were optimized, resulting in optimized values of 50 μm electrode gap, 80 μg/mL pEGFP-N1 concentration, 6 V pulse voltage, and 2-pulse per time with above 95% confidence. The results show that the gene transfection efficiency is highly enhanced due to the dramatically increased pEGFP-N1 concentration by the electrostatic force. With optimized factors, the pEGFP-N1 plasmid was successfully transfected into the basal cell carcinoma cell line (BCC) with 35.89 % average transfection rate.

原文English
主出版物標題Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
主出版物子標題9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
發行者Transducer Research Foundation
頁面1425-1427
頁數3
ISBN(列印)0974361119, 9780974361116
出版狀態Published - 2005 1月 1
事件9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
持續時間: 2005 10月 92005 10月 13

出版系列

名字Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
國家/地區United States
城市Boston, MA
期間05-10-0905-10-13

All Science Journal Classification (ASJC) codes

  • 化學工程(雜項)
  • 生物工程

指紋

深入研究「Optimization of an electroporation system for gene transfection by taguchi method」主題。共同形成了獨特的指紋。

引用此