TY - JOUR
T1 - Optimization of an enzyme linked DNA aptamer assay for cardiac troponin i detection
T2 - Synchronous multiple sample analysis on an integrated microfluidic platform
AU - Gopinathan, Priya
AU - Sinha, Anirban
AU - Chung, Yi Da
AU - Shiesh, Shu Chu
AU - Lee, Gwo Bin
N1 - Funding Information:
The authors would like to acknowledge financial support from Taiwan’s Ministry of Science and Technology (MOST 106-2221-E-007-001 and MOST 107-2314-B-007-005 to G. B. L.) Partial financial support from Taiwan’s National Health Research Institutes (NHRI-EX107-10728EI to G. B. L.) and the Ministry of Education of Taiwan’s Higher Education “Sprout” Project (grant 107Q2713E1 to G. B. L.) are also greatly appreciated.
Publisher Copyright:
© The Royal Society of Chemistry 2019.
PY - 2019/8/21
Y1 - 2019/8/21
N2 - In this study, an enzyme linked DNA aptamer based assay was optimized for human cardiac troponin I (cTnI) detection which is a prominent biomarker for acute myocardial infarction (AMI), on an integrated microfluidic platform. This platform allowed for the multiplex detection of six samples (5 μL per sample), and only 30 min were required for detection. First, cTnI-specific aptamers were surface-coated on magnetic beads. Bead-captured proteins were allowed to bind to a primary cTnI antibody and then to a secondary antibody labelled with horseradish peroxidase. Finally, chemiluminescence intensities were detected for quantification of cTnI. Purified proteins, serum from AMI patients and unknown serum samples were used to test the efficacy of the on-chip system. The limit of detection was measured to be only 12 ng L-1, and off-target effects from other proteins were minimal. This sensitive, cTnI-specific aptamer-based assay could consequently be used for reliable diagnosis of AMI.
AB - In this study, an enzyme linked DNA aptamer based assay was optimized for human cardiac troponin I (cTnI) detection which is a prominent biomarker for acute myocardial infarction (AMI), on an integrated microfluidic platform. This platform allowed for the multiplex detection of six samples (5 μL per sample), and only 30 min were required for detection. First, cTnI-specific aptamers were surface-coated on magnetic beads. Bead-captured proteins were allowed to bind to a primary cTnI antibody and then to a secondary antibody labelled with horseradish peroxidase. Finally, chemiluminescence intensities were detected for quantification of cTnI. Purified proteins, serum from AMI patients and unknown serum samples were used to test the efficacy of the on-chip system. The limit of detection was measured to be only 12 ng L-1, and off-target effects from other proteins were minimal. This sensitive, cTnI-specific aptamer-based assay could consequently be used for reliable diagnosis of AMI.
UR - http://www.scopus.com/inward/record.url?scp=85070293463&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85070293463&partnerID=8YFLogxK
U2 - 10.1039/c9an00779b
DO - 10.1039/c9an00779b
M3 - Article
C2 - 31317135
AN - SCOPUS:85070293463
SN - 0003-2654
VL - 144
SP - 4943
EP - 4951
JO - Analyst
JF - Analyst
IS - 16
ER -