Optimization of aptamer selection on an automated microfluidic system with cancer tissues

Cheng Sheng Lin, Yi Cheng Tsai, Keng Fu Hsu, Gwo Bin Lee

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

Cancer is among the world's most deadly inflictions, and early diagnosis is critical. Aptamers have shown utility as cancer probes since they can be screened rapidlyin vitroagainst cancer tissues using systematic evolution of ligands by exponential enrichment (SELEX) process. However, bench-top SELEX procedures are relatively labor-intensive and time-consuming; ideally, they could instead be carried out on microfluidic devices, yet this requires optimization of buffer and reaction conditions. Herein an integrated microfluidic system (IMS) was established to automatically carry out the optimization of aptamer selection. A “formulation chip” was developed that could mix salt solutions at differing final concentrations, and the resulting optimal binding buffer was transferred to another “optimization-SELEX chip” for the following tissue-SELEX. Two aptamers were successfully screened; one of which, H-45, exhibited high specificity and affinity towards ovarian cancer tissue samples, suggesting that this IMS might be a promising device for screening of cancer associated aptamers for cancer diagnosis.

原文English
頁(從 - 到)725-734
頁數10
期刊Lab on a Chip
21
發行號4
DOIs
出版狀態Published - 2021 二月 21

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物化學
  • 化學 (全部)
  • 生物醫學工程

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