TY - GEN
T1 - Optimization of gene transfection condition using taguchi method for an electroporation microchip
AU - Huang, Keng Shiang
AU - Yang, Sheng Chung
AU - Chen, Hung Yi
AU - Lin, Yu Cheng
PY - 2007/12/1
Y1 - 2007/12/1
N2 - This study has demonstrated the optimization of an electroporation (EP) system for gene transfection by using the Taguchi method. The parameters of the EP system, which could have an influence on the transfecting rate, were optimized, resulting in values of 50 μm electrode gap, 80 μg/mL pEGFP-Nl concentration, 6 V pulse voltage, and 2-pulse. A larger-the-better Ω transformation was employed for better additivity. The analyzed results of interaction A × C, A × D, and C × D showed that there were strong interactions on level 3 of factors A, C, and D to each others. Using optimized EP system, the pEGFP-Nl plasmid was successfully transfected into the basal cell carcinoma cell line (BCC) with 40.36% average transfecting rate.
AB - This study has demonstrated the optimization of an electroporation (EP) system for gene transfection by using the Taguchi method. The parameters of the EP system, which could have an influence on the transfecting rate, were optimized, resulting in values of 50 μm electrode gap, 80 μg/mL pEGFP-Nl concentration, 6 V pulse voltage, and 2-pulse. A larger-the-better Ω transformation was employed for better additivity. The analyzed results of interaction A × C, A × D, and C × D showed that there were strong interactions on level 3 of factors A, C, and D to each others. Using optimized EP system, the pEGFP-Nl plasmid was successfully transfected into the basal cell carcinoma cell line (BCC) with 40.36% average transfecting rate.
UR - http://www.scopus.com/inward/record.url?scp=50049088795&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50049088795&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2007.4300263
DO - 10.1109/SENSOR.2007.4300263
M3 - Conference contribution
AN - SCOPUS:50049088795
SN - 1424408423
SN - 9781424408429
T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 847
EP - 850
BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
Y2 - 10 June 2007 through 14 June 2007
ER -