Optimization of gene transfection condition using taguchi method for an electroporation microchip

Keng Shiang Huang, Sheng Chung Yang, Hung Yi Chen, Yu Cheng Lin

研究成果: Conference contribution

3 引文 斯高帕斯(Scopus)

摘要

This study has demonstrated the optimization of an electroporation (EP) system for gene transfection by using the Taguchi method. The parameters of the EP system, which could have an influence on the transfecting rate, were optimized, resulting in values of 50 μm electrode gap, 80 μg/mL pEGFP-Nl concentration, 6 V pulse voltage, and 2-pulse. A larger-the-better Ω transformation was employed for better additivity. The analyzed results of interaction A × C, A × D, and C × D showed that there were strong interactions on level 3 of factors A, C, and D to each others. Using optimized EP system, the pEGFP-Nl plasmid was successfully transfected into the basal cell carcinoma cell line (BCC) with 40.36% average transfecting rate.

原文English
主出版物標題TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
頁面847-850
頁數4
DOIs
出版狀態Published - 2007 12月 1
事件4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
持續時間: 2007 6月 102007 6月 14

出版系列

名字TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
國家/地區France
城市Lyon
期間07-06-1007-06-14

All Science Journal Classification (ASJC) codes

  • 控制與系統工程
  • 電氣與電子工程

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