TY - JOUR
T1 - Optimizing heterotrophic production of Chlorella sorokiniana SU-9 proteins potentially used as a sustainable protein substitute in aquafeed
AU - Chen, Chun Yen
AU - Lu, Jhih Ci
AU - Chang, Yu Han
AU - Chen, Jih Heng
AU - Nagarajan, Dillirani
AU - Lee, Duu Jong
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial support from the Taiwan Ministry of Science and Technology under grants MOST 111-3116-F-006 -004, 111-2621-M-029 -002, 110-2221-E-029 -004 -MY3, and 110-2622-E-110 -016.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2023/2
Y1 - 2023/2
N2 - Alternative protein sources for the reduction/replacement of fish meal in aqua-feeds are in urgent demand. Microalgae are considered sustainable protein sources for aquaculture due to their high-quality proteins with a complete profile of essential amino acids. This study examined the heterotrophic production of proteins from Chlorella sorokiniana SU-9. Culture parameters for maximal biomass and protein production are as follows: glucose – 10 g/L glucose, sodium nitrate – 1.5 g/L, and iron – 46 μM iron in BG-11 medium. Under optimal conditions, biomass content, protein content and protein productivity of SU-9 reached 4.14 ± 0.20 g/L, 403 ± 33 mg/g and 382 ± 36 mg/L/d, respectively. The protein profile of Chlorella sorokiniana SU-9 is comparable to fishmeal and soybean meal. The essential amino acids arginine, lysine and cysteine, along with glutamine and glutamate, were high. The production cost of SU-9 can be significantly reduced under heterotrophic cultivation conditions, making it a potential protein substitute in aquafeed.
AB - Alternative protein sources for the reduction/replacement of fish meal in aqua-feeds are in urgent demand. Microalgae are considered sustainable protein sources for aquaculture due to their high-quality proteins with a complete profile of essential amino acids. This study examined the heterotrophic production of proteins from Chlorella sorokiniana SU-9. Culture parameters for maximal biomass and protein production are as follows: glucose – 10 g/L glucose, sodium nitrate – 1.5 g/L, and iron – 46 μM iron in BG-11 medium. Under optimal conditions, biomass content, protein content and protein productivity of SU-9 reached 4.14 ± 0.20 g/L, 403 ± 33 mg/g and 382 ± 36 mg/L/d, respectively. The protein profile of Chlorella sorokiniana SU-9 is comparable to fishmeal and soybean meal. The essential amino acids arginine, lysine and cysteine, along with glutamine and glutamate, were high. The production cost of SU-9 can be significantly reduced under heterotrophic cultivation conditions, making it a potential protein substitute in aquafeed.
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U2 - 10.1016/j.biortech.2022.128538
DO - 10.1016/j.biortech.2022.128538
M3 - Article
C2 - 36581231
AN - SCOPUS:85145987574
SN - 0960-8524
VL - 370
JO - Bioresource technology
JF - Bioresource technology
M1 - 128538
ER -
