Optimizing proliferation and characterization of multipotent stem cells from porcine adipose tissue

Kai Hung Wang, An Pei Kao, Hsuan Wangchen, Fu Yuan Wang, Chih Hau Chang, Chia Cheng Chang, Sin Daw Lin

研究成果: Article同行評審

25 引文 斯高帕斯(Scopus)

摘要

Porcine mesenchymal stem cells have been isolated previously from bone marrow but not from adipose tissue. In the present study a new cell-culture method, using a low-calcium medium supplemented with N-acetyl-L-cysteine and L-ascorbic acid 2-phosphate (the PM2 medium) was developed to grow pASCs (porcine adipose-tissue-derived stem cells). The pASCs developed using the new medium showed a high growth rate and a high proliferation potential, as measured by a cumulative population doubling level (55) that was significantly higher than those reported for ASCs in the literature. These pASCs lacked gap-junctional intercellular communication and were capable of differentiation into three mesodermal lineages (i.e. adipocytes, osteoblasts and chondrocytes) and an ectodermal lineage (i.e. neural cells). Surprisingly, osteogenic ability, but not adipogenesis, was found to increase dramatically with increasing passages. The high proliferative and differentiation potential of these pASCs should facilitate the development of a large-animal model to study the use of ASCs in regenerative and reparative medicine.

原文English
頁(從 - 到)159-166
頁數8
期刊Biotechnology and Applied Biochemistry
51
發行號4
DOIs
出版狀態Published - 2008 12月

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 生物工程
  • 分子醫學
  • 生物醫學工程
  • 應用微生物與生物技術
  • 藥物發現
  • 製程化學與技術

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